Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-604-I Anti-Rac2 Antibody

Detect Rac2 using this rabbit polyclonal Anti-Rac2, Cat. No. 07-604-I, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Rac2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: RAC2 UniProt Number: P15153
07-604-I
100 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-604-I
Replaces07-604
DescriptionAnti-Rac2 Antibody
Alternate Names
  • Ras-related C3 botulinum toxin substrate 2
  • GX
  • p21-Rac2
  • Small G protein
Background InformationRas-related C3 botulinum toxin substrate 2 (UniProt P15153; also known as GX, p21-Rac2, Small G protein) is encoded by the RAC2 gene (Gene ID 5880) in human. Rho family of small GTPases are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Rac1 and Rac2 share high sequence homology (90%) and are both expressed in the hematopoietic system, where they play crucial roles in mediating the interactions between hematopoietic stem cells (HSCs) and their microenvironment. Rac proteins transduce cell surface signaling events initiated by tyrosine kinase receptors, G protein-coupled receptors, and cell-to-cell contacts by acting as molecular switches, cycling between an inactive GDP-bound state and a GTP-bound active state. In addition to overlapping functions, Rac1 and Rac2 also mediate unique functions via interactions with specific partners. SAM50 and Metaxin1, for example, are among mitochondrial proteins identified to be specifically associated with Rac2. Rac activity inhibition is reported to effectively target primary human AML and CML cells and prevent disease initiation in various murine leukemia models.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Rac2 using this rabbit polyclonal Anti-Rac2, Cat. No. 07-604-I, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Rac2 in human tonsil, human small intestine, and mouse spleen tissue sections.
Biological Information
ImmunogenGST-tagged recombinant full-length human Rac2 pro-form.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody was raised against recombinant full-length human Rac2 pro-form (a.a. 1-192) and therefore will recognize both the pro- and mature (a.a. 1-189) Rac2.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAC2
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~21 kDa observed. 21.12/21.43 kDa (human mature/pro-form) and 21.13/21.44 kDa (mouse mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Rac2 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
07-604-I 04054839090219