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MABS2314-100UG Anti-RHBDF2 Antibody, clone RHF2B 11H7

Anti-RHBDF2, clone RHF2B 11H7, Cat. No. MABS2314, Is a rat monoclonal antibody that detects RHBDF2 and is tested for use in Western Blotting.

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Description
Catalogue NumberMABS2314-100UG
DescriptionAnti-RHBDF2 Antibody, clone RHF2B 11H7
Alternate Names
  • inactive rhomboid protein 2
  • iRhom2
  • Rhomboid family member 2
  • Rhomboid veinlet-like protein 6
  • Rhomboid-related protein
Background InformationInactive rhomboid protein 2 (UniProt: Q80WQ6; also known as iRhom2, Rhomboid family member 2, Rhomboid veinlet-like protein 6, Rhomboid-related protein) is encoded by the Rhbdf2 (also known as Irhom2, Rhbdl6, Rhor) in murine species. RHBDF2/iRhom2, a multi-pass membrane protein with seven transmembrane domains, is predominantly localized in the endoplasmic reticulum (ER) membrane. It is reported to be crucial for the maturation and function of ADAM17 in myeloid cells. Hence, mice lacking this protein are protected from ADAM17- and TNF -dependent septic shock and inflammatory arthritis. ADAM17, a sheddase of the EGF receptor ligands and TNF , is shown to be involved in cell survival, proliferation, migration, and inflammation. However, it does not exhibit any protease activity on its own. RHBDF2 is involved in negative regulation of protein secretion and regulation of EGF receptor signaling pathway. By interacting with EGF, it may retain EGF in the endoplasmic reticulum and regulate its degradation through the ER-associated degradation (ERAD). (Ref.: Weskamp, G., et al. (2020). J. Biol. Chem. 295(13); 4350-4358; Li, X., et al. (2017). J. Cell Sci. 130(5); 868-878; Blaydon, DC., et al. (2012). Am. J. Hum. Genet. 90(2); 340-436; Zetti, M., et al. (2011). Cell. 145(1); 79-91).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-RHBDF2, clone RHF2B 11H7, Cat. No. MABS2314, Is a rat monoclonal antibody that detects RHBDF2 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
  • Western Blot Using KO Lysates
Application NotesTested Applications

Western Blotting:

Lipopolysaccharide (LPS; 100 ng/mL; 24 h)-treated wild-type macrophage fractions from mouse bone marrow and RHBDF2 knockouts were probed with Cat. No. MABS2314, Anti-RHBDF2, clone RHF2B 11H7 (1:500 Dilution). Proteins were visualized using a Goat Anti-Rat IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RHBDF2 (~ 98 kDa). (Data courtesy: Katrin Moschke, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Munich.

Western Blotting Analysis: A representative lot detected RHBDF2 in Western Blotting applications (Weskamp, G., et al. (2020). J Biol Chem. 295(13):4350-4358).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of mouse Inactive rhomboid protein 2 (RHBDF2).
EpitopeN-terminal half
CloneRHF2B 11H7
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone RHF2B 11H7 is a rat monoclonal antibody that detects Inactive rhomboid protein 2 (RHBDF2). It targets an epitope within 15 amino acids from the N-terminal half.
IsotypeIgG2a
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rhbdf2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~95 kDa observed; 93.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RHBDF2/iRhom2 in MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS2314-100UG 04065270845344