Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN1855-25UL Anti-RGS14 Antibody, clone N133/35

Anti-RGS14, clone N133/35, Cat. No. MABN1855, is a mouse monoclonal antibody that detects G-protein signaling 14 and is tested for use in Western Blotting.

MABN1855-25UL
25 µL  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Catalogue NumberMABN1855-25UL
DescriptionAnti-RGS14 Antibody, clone N133/35
Alternate Names
  • Regulator of G-protein signaling 14
Background InformationRegulator of G-protein signaling 14 (UniProt: O08773; also known as RGS14) is encoded by the Rgs14 gene (Gene ID: 114705) in rat. RGS14 is expressed at high levels in the brain cortex, hippocampus, striatum, thalamus and substantia nigra. Its expression has also been reported in the lung, and spleen. It regulates G protein-coupled receptor signaling cascades and inhibits signal transduction by increasing the GTPase activity of G protein a subunits, thereby driving them into their inactive GDP-bound form. It also serves as a GDP-dissociation inhibitor (GDI) and thereby modulates signal transduction via G protein a subunits. It is reported to display GDI activity on Gi a subunits GNAI1 and GNAI3, but not on GNAI2 and Go a subunit GNAO1. RGS14 also serves as a positive modulator of microtubule polymerization and spindle organization through a Gi a -dependent mechanism. It is reported to associate with the perinuclear sheaths of microtubules surrounding the pronuclei, prior to segregating to the anastral mitotic apparatus. RGS14 contains a RGS domain (aa 67-184) that is essential for its GTPase-activating protein (GAP) activity for G subunits and localization to the nucleus and centrosomes and its GoLoco domain (aa 497-519) is shown to be necessary for its GDI activity and, translocation out of the nucleus and interaction with GNAI1, GNAI2 and GNAI3. It also has two RBD domains (aa 300-371 and 373-443) that are also essential for its localization to the nucleus and centrosomes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-RGS14, clone N133/35, Cat. No. MABN1855, is a mouse monoclonal antibody that detects G-protein signaling 14 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected RGS14 in mouse hippocampus tissue lysate.
Biological Information
ImmunogenFull-length recombinant rat Regulator of G-protein signaling 14 (RGS14).
CloneN133/35
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone N133/35 is a mouse monoclonal antibody that detects Regulator of G-protein signaling 14 (RGS14).
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rgs14
Purification MethodProtein G purified
UniProt Number
Molecular Weight~60 kDa observed; 59.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat hippocampus tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RGS14 in Rat hippocampus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABN1855-25UL 04061841762096