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MABC1690 Anti-RAINBOW

MABC1690
100 µL  
Purchase on Sigma-Aldrich

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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1690
DescriptionAnti-RAINBOW
Background InformationPre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl, pH 7.4, with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RAINBOW, Cat. No. MABC1690, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Remazol-dye stained protein markers in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Western Blotting Analysis: A 1:250 and 1:500 dilution from a representative lot detected Remazol-dye Pre-Stained protein standards.
Biological Information
ImmunogenRemazol-dyes phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityAnti-RAINBOW is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Pre-Stained protein standard.

Western Blotting Analysis: A 1:1,000, 1:5,000, and 1:10,000 dilution of this antibody detected Remzaol-dye prestained protein standards.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABC1690 04054839324338