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AB5925 Anti-Protein Gene Product 9.5 Antibody

AB5925
50 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Po, RWB, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberAB5925
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Protein Gene Product 9.5 Antibody
Alternate Names
  • PGP 9.5
  • UCH-L1
Product Information
FormatSerum
PresentationLiquid with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Protein Gene Product 9.5 using this Anti-Protein Gene Product 9.5 Antibody validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blot: 1:1,000
Immunohistochemistry: 1:2,000
Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 108-127 of soluble cytoplasmic human PGP9.5.
HostRabbit
SpecificityProtein Gene Product 9.5 (PGP9.5).
Species Reactivity
  • Human
  • Mouse
  • Pig
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryUCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]
Gene Symbol
  • UCHL1
  • PARK5
  • UCH-L1
  • Uch-L1
  • PGP9.5
  • EC 3.4.19.12
  • EC 6.-.-.-
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09936 # Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
SIZE: 223 amino acids; 24824 Da
SUBUNIT: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary.
PTM: O-glycosylated (By similarity).
DISEASE: SwissProt: P09936 # Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.
SIMILARITY: SwissProt: P09936 ## Belongs to the peptidase C12 family.
MISCELLANEOUS: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Global Trade Item Number
Katalogové číslo GTIN
AB5925 04053252281624