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MABN864 Anti-Potassium Channel Kir4.1 Antibody, clone 9C9.2

Anti-Potassium Channel Kir4.1 Antibody, clone 9C9.2 is an antibody against Potassium Channel Kir4.1 for use in Western Blotting, Immunohistochemistry (Paraffin).

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Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNJ10, SESAMES UniProt Number: P78508
MABN864
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN864
DescriptionAnti-Potassium Channel Kir4.1 Antibody, clone 9C9.2
Alternate Names
  • ATP-sensitive inward rectifier potassium channel 10
  • ATP-dependent inwardly rectifying potassium channel Kir4.1
  • Inward rectifier K(+) channel Kir1.2
  • Potassium channel, inwardly rectifying subfamily J member 10
Background InformationATP-sensitive inward rectifier potassium channel 10 (UniProt P78508; also known as ATP-dependent inwardly rectifying potassium channel Kir4.1, Inward rectifier K(+) channel Kir1.2, Potassium channel, inwardly rectifying subfamily J member 10) is encoded by the KCNJ10 (also known as SESAMES) gene (Gene ID 3766) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs, and ATP-sensitive Kirs. Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. Kir4.1 is widely expressed in the kidney distal convoluted tubules and neural glia cells, its expression in the cochlea and retina is also reported. Kir4.1 modulates plasma K+ by favoring K+ exit, and inhibition of Kir4.1 results in membrane depolarization. KCNJ10 gene mutations are known causes of SeSAMES (seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance syndrome). Human Kir4.1 is a 379-amino acid multi-pass membrane (a.a. 65-89, 115-126, 143-164) protein containg two small extraceullar regions (a.a. 90-114 and 134-142), a pore-forming domain (a.a. 134-142), and having both N- and C-terminal exposed intracellularly (a.a. 1-64 and a.a. 165-379).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Potassium Channel Kir4.1 Antibody, clone 9C9.2 is an antibody against Potassium Channel Kir4.1 for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected potassium channel Kir4.1 in 10 µg of mouse cerebellum tissue lysate.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected potassium channel Kir4.1 immunoreactivity in the molecular layer of human cerebellum tissue sections.
Immunohistochemistry Analysis: A 1:25 dilution from a representative lot immunostained glial cells near ventricles in rat brain and astrocytes in the white matter of human cerebellum tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptides corresponding to sequences from the first extracellular domain and the C-terminal cytoplasmic tail of human potassium channel Kir4.1.
Clone9C9.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9C9.2 detects potassium channel Kir4.1.
IsotypeIgMκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KCNJ10
  • SESAMES
UniProt Number
Molecular Weight~49 kDa observed. Target band size appears larger than the calculated molecular weight of 42.51 kDa due to glycosylation. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting Analysis in mouse brain tissue lysate:

Western Blotting Analysis: 2.0 µg/mL of this antibody detected potassium channel Kir4.1 in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABN864 04055977332216