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05-777-AF647 Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647

Anti-Phosphotyrosine Antibody, clone 4G10, Alexa Fluor® 647 is a highly validated recombinant mouse monoclonal antibody that targets protein tyrosine phosphorylation and has been tested in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling
05-777-AF647
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
AICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue Number05-777-AF647
DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647
Background InformationPhosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphotyrosine Antibody, clone 4G10, Alexa Fluor® 647 is a highly validated recombinant mouse monoclonal antibody that targets protein tyrosine phosphorylation and has been tested in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesThis recombinant monoclonal antibody is also available as HRP conjugate (Cat. No. 16-184), agarose conjugate (Cat. No. 16-199), biotin conjugate (Cat. No. 16-204), FITC conjugate (Cat. No. 16-205), PE conjugate (Cat. No. FCMAB323PE), as well as as unconjugated antibody (Cat. No. 05-777) for immunoaffinity purification, flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.
Biological Information
ImmunogenProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor® 647.
Clone4G10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
Species Reactivity
  • All
Species Reactivity NoteAll. Target modification is not species-specific.
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G purified.
Molecular WeightVariable depending on the size(s) of the tyrosine-phosphorylated protein(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected EGF-induced tyrosine phosphorylation of cellular proteins in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
05-777-AF647 04054839034619

Documentation

Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647 Certificates of Analysis

TitleLot Number
Anti-Phosphotyrosine, recombinant clone 4G10®, Alexa Fluor® 647 - 3278573 3278573
Anti-Phosphotyrosine, recombinant clone 4G10®, Alexa Fluor® 647 - 3885480 3885480
Anti-Phosphotyrosine, recombinant clone 4G10®, Alexa Fluor® 647 - 4069735 4069735
Anti-Phosphotyrosine, recombinant clone 4G10®, Alexa Fluor® 647 -Q2726971 Q2726971