Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PH03L Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D)

PH03L
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the T-cell PTPase protein.

Catalogue NumberPH03L
Brand Family Calbiochem®
SynonymsAnti-Protein Tyrosine Phosphatase, T cell
References
ReferencesBrown-Shimer, S., et al. 1992. Can. Res. 52, 478.
Fischer, E.H., et al. 1991 Science 253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
Positive controlCCRF-CEM cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunocytochemistry (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application CommentsThis antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human T cell PTPase
ImmunogenHuman
CloneCF4-1D
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PH03L 0

Documentation

Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) MSDS

Title

Safety Data Sheet (SDS) 

Anti-PTP, T Cell (Ab-1) Mouse mAb (CF4-1D) Certificates of Analysis

TitleLot Number
PH03L

References

Přehled odkazů
Brown-Shimer, S., et al. 1992. Can. Res. 52, 478.
Fischer, E.H., et al. 1991 Science 253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-October-2007 RFH
SynonymsAnti-Protein Tyrosine Phosphatase, T cell
ApplicationImmunocytochemistry (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the PTPase protein.
BackgroundProtein tyrosine phosphatases (PTPases) make up an expanding family of transmembrane and intracellular proteins, which function to remove phosphate groups from protein phosphoryl- tyrosine residues. The prototype phosphatase, PTPase 1B, was initially identified as an active intracellular phosphatase from human placenta made up of 321 amino acids and subsequently shown to derive from a larger precursor protein containing 435 amino acids. Using probes to the conserved PTPase domain a cDNA clone was isolated from a human peripheral T cell library encoding a protein with 65% identity to the placental PTPase 1B. The T cell protein differs in amino acid composition but is highly similar with respect to hydrophobicity with the 1B protein and is now known to be present in other cell types. While the function of the T cell PTPase protein is still unknown, recent experiments demonstrated that a truncated form overexpressed in BHK cells resulted in a greatly reduced growth rate of the cells. PTPases are involved in regulating processes where protein kinases and protein tyrosine phosphorylation are important. One intriguing hypothesis is that PTPases have the potential to function as anti-oncogenes.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human T cell PTPase
CloneCF4-1D
IsotypeIgG2a
Specieshuman, not mouse
Positive controlCCRF-CEM cells
FormLyophilized
FormulationLyophilized from 20 mM sodium bicarbonate, 100 µg BSA.
PreservativeNone
CommentsThis antibody has been reported to work for immunocytochemistry. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4 to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesBrown-Shimer, S., et al. 1992. Can. Res. 52, 478.
Fischer, E.H., et al. 1991 Science 253, 401.
Brown-Shimer, S., et al. Proc. Natl. Acad. Sci. USA 87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
Cool, D.E., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 7280.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
Cool, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5257.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.