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ABS2123 Anti-PPOX

Anti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.

Anti-PPOX MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PPOX UniProt Number: P50336
ABS2123
100 µL  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABS2123
DescriptionAnti-PPOX
Alternate Names
  • Protoporphyrinogen oxidase
  • PPOX
Background InformationProtoporphyrinogen oxidase (UniProt: P50336; also known as EC: 1.3.3.4, PPO, PPOX) is encoded by the PPOX gene (Gene ID: 5498) in human. Protoporphyrinogen oxidase is a mitochondrial inner membrane enzyme that catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. It is involved in step one of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas and may be present as a monomer or active homodimeric form. Defects in PPOX are linked to variegate porphyria that lead to severe deficiency of this enzyme that result in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected PPOX in undifferentiated (day 0) and maturing (day 3 or 4) mouse erythroleukemia (MEL) cells (Chung, J., et. al. (2015). Sci Signal. 8(372):ra34). and differentiated Mouse erythroleukemia (MEL) cells (Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).
Biological Information
ImmunogenFull length recombinant human Protoporphyrinogen oxidase (PPOX).
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Protoporphyrinogen oxidase (PPO) in Human and Mouse cells.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PPOX
Purification MethodUnpurified
UniProt Number
Molecular Weight~53 kDa observed; 50.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lystate from mitochondria isolated from differentiated DS19 cells.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABS2123 04054839328855