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MABE1003 Anti-PIF1 Antibody, clone 12A11.1

Anti-PIF1 Antibody, clone 12A11.1 is an antibody against PIF1 for use in Western Blotting.

Anti-PIF1 Antibody, clone 12A11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: PIF1, C15orf20 UniProt Number: Q9H611
MABE1003
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1003
DescriptionAnti-PIF1 Antibody, clone 12A11.1
Alternate Names
  • ATP-dependent DNA helicase PIF1
  • PIF1
  • DNA repair and recombination helicase PIF1
  • PIF1/RRM3 DNA helicase-like protein
Background InformationATP-dependent DNA helicase PIF1 (EC 3.6.4.12; UniProt Q9H611; also known as DNA repair and recombination helicase PIF1, Petite integration frequency 1, PIF1 5'-to-3' DNA helicase homolog, PIF1/RRM3 DNA helicase-like protein) is encoded by the PIF1 (also known as PIF, C15orf20) gene (Gene ID 80119) in human. Human PIF1 helicase and the yeast ortholog share common substrates in vitro, exhibiting specificity toward telomeric DNA, synthetic stalled DNA replication fork-like structures, especially G4-DNA. PIF1 functions to maintain undisrupted DNA replication fork progression during normal cycling conditions and to support resumption of DNA synthesis after prolonged S-phase arrest. Cancer cells are reported to exhibit increased dependency on PIF for DNA replication. PIF1-depletion results in a combination of apoptosis, reduced survival, and hypersensitivity to therapeutic DNA replication inhibitors in several cancer cell lines independent of p53 status.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PIF1 Antibody, clone 12A11.1 is an antibody against PIF1 for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminal half of human PIF1.
EpitopeC-terminal half
Clone12A11.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with isoforms 1 (isoform alpha) and isoform 4, but not isoform 2. Reactivity toward isoform 3 (isoform beta) has not been determined.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PIF1
  • C15orf20
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~70 kDa observed. 69.8 kDa (isoform 1), 77.1 kDa (isoform 3), 63.9 kDa (isoform 4) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PIF1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE1003 04055977297379