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PC474 Anti-PCNA (247-261) (Ab-5) Rabbit pAb

This Anti-PCNA (247-261) (Ab-5) Rabbit pAb is validated for use in Immunoprecipitation for the detection of PCNA (247-261) (Ab-5).

Anti-PCNA (247-261) (Ab-5) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Proliferating Cell Nuclear Antigen

PC474
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Přehled

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Katalogové čísloBalení ks/bal.
PC474-50UL Plastová ampulka 50 ul
Přidat k oblíbeným položkám
Description
OverviewRecognizes the ~37 kDa PCNA protein in MCF-7 cells.
Catalogue NumberPC474
Brand Family Calbiochem®
SynonymsAnti-Proliferating Cell Nuclear Antigen
Application Data
Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
References
ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlMCF7 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (1:250-1:1000)
Application CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Katalogové číslo GTIN
PC474-50UL 04055977208337