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MAB2014 Anti-OB-Cadherin Antibody, extracellular domain, clone 16A

Detect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH.

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Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CDH11, CDHOB, CAD11, OB, cadherin-11, OSF-4, OB-cadherin UniProt Number: P55287
MAB2014
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2014
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-OB-Cadherin Antibody, extracellular domain, clone 16A
Alternate Names
  • Cadherin-11
Background InformationCadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
References
Product Information
FormatPurified
PresentationAffinity purified immuoglobulin. Liquid in PBS containing 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect OB-Cadherin using this Anti-OB-Cadherin Antibody, extracellular domain, clone 16A validated for use in WB, IC, IH.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesWestern blot: 1:25 - 1:250
Immunohistochemistry: Frozen tissue sections. 1:25 - 1:50 dilution using avidin-biotinylated horseradish peroxidase complex (ABC) as detection protocol.
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells
Optimal working dilutions must be determined by the end user.I/
Biological Information
ImmunogenFusion protein corresponding to extracellular domain of human OB-cadherin coupled to GST.
Epitopeextracellular domain
Clone16A
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the extracellular domain of human cadherin-11.
IsotypeIgG1
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.
Gene Symbol
  • CDH11
  • CDHOB
  • CAD11
  • OB
  • cadherin-11
  • OSF-4
  • OB-cadherin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P55287 # Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
SIZE: 796 amino acids; 87965 Da
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts.
SIMILARITY: SwissProt: P55287 ## Contains 5 cadherin domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 4°C from date of shipment
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MAB2014 04053252398889