Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN281 Anti-Numb Antibody, clone 6G10.1

Detect Numb using this Anti-Numb Antibody, clone 6G10.1 validated for use in WB & IC.

Anti-Numb Antibody, clone 6G10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: NUMB UniProt Number: P49757
MABN281
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN281
DescriptionAnti-Numb Antibody, clone 6G10.1
Alternate Names
  • Protein numb homolog
  • h-Numb
  • S171
Background InformationNumb plays a key role in neurogenesis and in cell fate decisions. It maintains self-renewal properties in neural progenitor cells, which are also called radial glial cells (RGCs). It functions through inhibition of Notch signaling. Additionally, it is suggested to mediate local repair in brain ventricular wall damage.
Product Information
FormatPurified
Control
  • A431 cell lysate
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Numb using this Anti-Numb Antibody, clone 6G10.1 validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Numb in HeLa and A431 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Numb isoforms 1 and 2.
Clone6G10.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes Numb isoforms 1 and 2.
IsotypeIgMκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • NUMB
UniProt Number
UniProt SummaryFUNCTION: Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

SUBUNIT STRUCTURE: Interacts with CDH1 and TFAP2B. Interacts with EPS15, LNX and NOTCH1. May interact with DUOXA1. Interacts with RALBP1 in a complex also containing EPN1 and TFAP2A during interphase and mitosis.

SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
Molecular Weight~70 kDa observed. Uniprot describes four isoforms at ~71 kDa (isoform 1), ~66 kDa (isoform 2), ~69 kDa (isoform 3), and ~65 kDa (isoform 4).
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Numb in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
MABN281 04053252437359