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ST1117 Anti-Nestin Rabbit pAb

Anti-Nestin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NES

ST1117
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~220 kDa Nestin protein in SHSY-5Y cells.
Catalogue NumberST1117
Brand Family Calbiochem®
SynonymsAnti-NES
Application Data
Lane 1: Detection of human nestin by immunoblotting. Sample: Whole cell lysate (20 µg) from SHSY-5Y cells. Primary antibody: Anti-Nestin Rabbit pAb (Cat. No ST1117) (0.5 µg/ml). Detection: chemiluminescence. Lanes 2 and 3: Detection of human nestin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from (500 µg) from SHSY-5Y cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-Nestin Rabbit pAb (Cat. No. ST1117) (5 µg). Immunoblotting conditions: same as lane 1.
References
ReferencesShih, A.H. and Holland, E.C. 2006. Neoplasia 8, 1072.
Singh, S.K., et al. 2004. Oncogene 23, 7267.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlSHSY-5Y cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (0.1 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids from an internal region of human NES
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1117 0

Documentation

References

Přehled odkazů
Shih, A.H. and Holland, E.C. 2006. Neoplasia 8, 1072.
Singh, S.K., et al. 2004. Oncogene 23, 7267.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-April-2008 JSW
SynonymsAnti-NES
ApplicationImmunoblotting (0.1 µg/ml)
Immunoprecipitation (5 µg/500 µg total protein)
Application Data
Lane 1: Detection of human nestin by immunoblotting. Sample: Whole cell lysate (20 µg) from SHSY-5Y cells. Primary antibody: Anti-Nestin Rabbit pAb (Cat. No ST1117) (0.5 µg/ml). Detection: chemiluminescence. Lanes 2 and 3: Detection of human nestin by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from (500 µg) from SHSY-5Y cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-Nestin Rabbit pAb (Cat. No. ST1117) (5 µg). Immunoblotting conditions: same as lane 1.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~220 kDa Nestin protein.
BackgroundNestin (accession no. NP_006608) is a large cytoplasmic intermediate filament protein that is a marker for neuroepithelial stem cells. Initially, nestin is expressed in neural stem cells and its expression is downregulated upon cell differentiation in astrocytes and neurons. Nestin has been used to identify stem cells in brain tumors and in malignant melanomas.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids from an internal region of human NES
IsotypeIgG
Specieshuman
Positive controlSHSY-5Y cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesShih, A.H. and Holland, E.C. 2006. Neoplasia 8, 1072.
Singh, S.K., et al. 2004. Oncogene 23, 7267.