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NB21 Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-NSF

NB21
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~76 kDa NSF-1 protein in human fibroblasts, MDBK, and PC12 cells.
Catalogue NumberNB21
Brand Family Calbiochem®
SynonymsAnti-NSF
References
ReferencesSutton, R.B., et al. 1998. Nature 395, 347.
Lemons, P.P., et al. 1997. Blood 90, 1490.
Edelmann, L., et al. 1995. EMBO J. 14, 224.
Südhof, T.C. 1995. Nature 375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol. 126, 945.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
Positive controlHuman or rat fibroblasts, MDBK cells, or PC12 cells
Preservative3 mM sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (1:2000)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
Application CommentsThis antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human N-ethylmaleimide-senstive factor
ImmunogenHuman
CloneNSF-1
HostMouse
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
NB21 0

Documentation

Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1) MSDS

Title

Safety Data Sheet (SDS) 

Anti-N-Ethylmaleimide-Sensitive Factor Mouse mAb (NSF-1) Certificates of Analysis

TitleLot Number
NB21

References

Přehled odkazů
Sutton, R.B., et al. 1998. Nature 395, 347.
Lemons, P.P., et al. 1997. Blood 90, 1490.
Edelmann, L., et al. 1995. EMBO J. 14, 224.
Südhof, T.C. 1995. Nature 375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol. 126, 945.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
SynonymsAnti-NSF
ApplicationELISA (1:2000)
Immunoblotting (1:2000)
Immunoprecipitation (1:500)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~76 kDa NSF-1 protein.
BackgroundSynaptic vesicle proteins can be divided into one of two classes; transport proteins involved in neurotransmitter uptake, and trafficking proteins which perform functions in vesicle docking, fusion, and budding. For example, synaptophysin may function in the process of neurotransmitter release, while synaptobrevin (VAMP) is believed to play a role in synaptic vesicle docking and exocytotic membrane fusion. Synaptobrevin has also been shown to bind synaptophysin with high specificity. Synapsins are highly abundant vesicle membrane proteins that are believed to regulate neurotransmitter release through phosphorylation-dependent interactions with cytoskeletal elements. Other proteins involved in vesicle exocytosis include syntaxin 1 and SNAP-25. In conjunction with synaptobrevin, these proteins form the SNARE (SNAP receptor) complex, which recruits other cytosolic factors including SNAP proteins (soluble NSF attachment proteins) and NSF (N-ethylmaleimide-sensitive factor). NSF acts in concert with SNAPs and SNAREs for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicle-to-plasma membrane transport. In its functional form, NSF is a homotrimer of 76 kDa subunits. Each subunit contains an N-terminal domain and two homologous ATP binding domains (D1 and D2). Vesicle-to-target membrane docking occurs when the vesicle SNARE binds to its cognate target membrane SNARE. SNAPs α and γ then bind to this docking complex and mediate the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human N-ethylmaleimide-senstive factor
CloneNSF-1
IsotypeIgG
Specieshuman, rat
Positive controlHuman or rat fibroblasts, MDBK cells, or PC12 cells
FormLiquid
FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative3 mM sodium azide
CommentsThis antibody is suitable for ELISA and immunoprecipitation under both native and denaturing conditions. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesSutton, R.B., et al. 1998. Nature 395, 347.
Lemons, P.P., et al. 1997. Blood 90, 1490.
Edelmann, L., et al. 1995. EMBO J. 14, 224.
Südhof, T.C. 1995. Nature 375, 645.
Whiteheart, S.W., et al. 1994. J. Cell Biol. 126, 945.