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05-915-25UL Anti-N-Cadherin Antibody, clone 13A9

Detect N-cadherin using this Anti-N-Cadherin Antibody, clone 13A9 validated for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-N-Cadherin Antibody, clone 13A9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CDH2, CDHN, NCAD UniProt Number: P19022
05-915-25UL
25 µL  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IHC, IP, WBMCulture SupernatantMonoclonal Antibody
Description
Catalogue Number05-915-25UL
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-N-Cadherin Antibody, clone 13A9
Alternate Names
  • Cadherin-2
  • CD325
  • CDw325
  • N-cadherin
  • Neural cadherin
Background InformationCadherin-2 (UniProt P19022; also known as CD325, CDw325, N-cadherin, Neural cadherin) is encoded by the CDH2 (also known as CDHN, NCAD) gene (Gene ID 1000) in human. Cadherins constitute a family of calcium-dependent cell-cell adhesion proteins that play important roles in the embryonic development and maintenance of normal tissue architecture. Cadherins are composed of an extracellular domain (a.a. 160-724 of human N-cadherin) with five homologous repeats that mediates adhesion, a single pass transmembrane domain (a.a. 725-745 of human N-cadherin), and a conserved cytoplasmic domain (a.a. 746-906 of human N-cadherin) that interacts with catenins to link cadherins to the actin cytoskeleton. In addition, a known Src substrate p120ctn also modulate the strength of cadherin-dependent adhesion by interacting with cadherins at their intracellular juxtamembrane domain. Cadherins are synthesized as precursor proteins that must be proteolytically cleaved to generate functional, mature proteins. Newly synthesized proN-cadherin (a.a. 1-906) is phosphorylated and proteolytically processed prior to transport to the plasma membrane. In addition, Plakoglobin (gamma-catenin) and beta-catenin associate only with phosphorylated proN-cadherin, whereas p120ctn can associate with both phosphorylated and non-phosphorylated proN-cadherin. The N-terminal signal and propeptide (a.a. 1-25 and 26-159 of of human N-cadherin) region is proteolytically removed and a core N-cadherin-catenin complex is assembled in the endoplasmic reticulum or Golgi compartment prior to localization at the plasma membrane where linkage to the actin cytoskeleton can be established.
References
Product Information
FormatCulture Supernatant
Control
  • HeLa cell lysate
PresentationMouse monoclonal immunoglobulin hybridoma culture supernatant containing 0.05% sodium azide before the addition of glycerol to 30%.
Quality LevelMQ100
Applications
ApplicationDetect N-cadherin using this Anti-N-Cadherin Antibody, clone 13A9 validated for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot immunostained the extracellular matrix of the stable plaques and in the fibrous cap region rich in vascular smooth muscle cells (VSMCs) using patients-derived paraffin-embedded internal carotid artery tissue sections (Musumeci, G., et al. (2014). Histol. Histopathol. 29(6):707-719).
Immunohistochemistry Analysis: A representative lot detected strong N-cadherin immunoreactivity in paraffin-embedded rectal cancer (RC) tissues with positive regional lymph node metastasis (RLNM) status, while only weak N-cadherin immunoreactivity was detected in RC with negative RLNM, and no N-cadherin staining was seen in normal colorectal epithelium (Fan, X.J., et al. (2012). Br. J. Cancer. 106(11):1735-1741).
Immunohistochemistry Analysis: A representative lot detected N-cadherin immunoreactivity in formalin-fixed, paraffin-embedded hepatocellular carcinoma (HCC) tissue sections. A significant inverse correlation was found between RUNX3 and N-cadherin expression levels (Tanaka, S., et al. (2012). Int. J. Cancer. 131(11):2537-2546).
Western Blotting Analysis: A representative lot detected an upregulated N-cadherin expression in CCL185 carcinoma cells following transient Epstein-Barr virus (EBV) infection. The EMT-like phenotype remained even after viral loss by culture selection pressure withdrawal (Queen, K.J., et al. (2013). Int. J. Cancer. 132(9):2076-2086).
Western Blotting Analysis: A representative lot detected N-cadherin in Hep3B, Huh7, HLF and SK-Hep1 human hepatocellular carcinoma (HCC) cell lysates (Tanaka, S., et al. (2012). Int. J. Cancer. 131(11):2537-2546).
Western Blotting Analysis: A representative lot detected both the unprocessed (pro-) and processed (mature) forms of N-cadherin in HeLa cell lysate (Wahl, J.K. 3rd., et al. (2003). J. Biol. Chem. 278(19):17269-17276).
Western Blotting Analysis: A representative lot detected N-cadherin in WI-38 human fibroblast lysate, but not in JAr human placental choriocarcinoma cell lysate (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
Immunocytochemistry Analysis: A representative lot detected N-cadherin immunoreactivity localized primarily at the cell-cell borders by fluorescent immunocytochemistry staining of 1% paraformaldehyde-fixed, methanol-permeabilized HeLa cells (Wahl, J.K. 3rd., et al. (2003). J. Biol. Chem. 278(19):17269-17276).
Immunocytochemistry Analysis: A representative lot detected N-cadherin immunoreactivity colocalized with those of alpha- and beta-catenin by dual fluorescent immunocytochemistry staining of fixed WI-38 human fibroblasts (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
Immunoprecipitation Analysis: Representative lots co-immunoprecipitated alpha-catenin, beta-catenin, and plakoglobin with N-cadherin from WI-38 human fibroblast and HeLa cell lysates (Wahl, J.K. 3rd., et al. (2003). J. Biol. Chem. 278(19):17269-17276; Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
Biological Information
ImmunogenBacterially expressed human N-cadherin cytoplasmic domain MBP fusion protein (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
EpitopeCytoplasmic domain.
Clone13A9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 13A9 recognizes N-cadherin, but not P-, E-, or M-cadherin (Knudsen, K.A., et al. (1995). J. Cell Biol. 130(1):67-77).
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDH2
  • CDHN
  • NCAD
Purification MethodUnpurified
UniProt Number
Molecular Weight~140 kDa observed. Target band size appears larger than the calculated molecular weights of 82.03 kDa (mature) and 99.81/97.04 kDa (isoform 1/2 pro-form) due to posttranslational glycosylation and phosphorylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1000-5000 dilution of this hybridoma culture supernatant detected N-cadherin in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
05-915-25UL 04054839342806