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475923 Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-M1

475923
  
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Tabulka spec. kláve

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Description
Overview

This product has been discontinued.





Recognizes the ~120 kDa muscarinic M1 acetylcholine receptor protein in rat brain. Supplied with a control peptide.
Catalogue Number475923
Brand Family Calbiochem®
SynonymsAnti-M1
Application Data
Detection of rat muscarinic M1 acetylcholine receptor by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the fusion protein antigen (lane 2). Primary antibody: Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb (Cat. No. 475923) (1:200). Detection: chemiluminescence.
References
ReferencesLevey, A.I., et al. 1991. J. Neurosci. 11, 3218.
Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
Smith. D.B., et al. 1988. Gene 67, 31.
Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 60 µg lyophilized control protein.
Positive controlM1-GST fusion protein (supplied) or rat brain
Preservative≤0.1% sodium azide (antibody only)
Applications
Application ReferencesImmunohistochemistry, Immunoprecipitation Levey, A.I., et al. 1991. J. Neurosci. 11, 3218. Immunoprecipitation Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:200-1:300, chemiluminescence)
Immunohistochemistry (see application references)
Immunoprecipitation (see application references)
Application CommentsThe epitope is specific for M1 and is not similar to any other known protein. To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. For use as a positive control in immunoblotting, use 10 ng/lane of the M1-GST fusion protein (minigel). For use as a negative control, preincubate 3-5 µg of M1-GST fusion protein with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena recombinant protein consisting of amino acids 227-353 of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor fused to GST
ImmunogenHuman
HostRabbit
IsotypeIgG
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl dH₂O to yield a stock concentration of 300 µg/ml. For long-term storage, aliquot the antibody and freeze (-20°); for short-term storage, refrigerate (4°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the M1-GST fusion protein with 100 µl H₂O, aliquot, and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
475923 0

Documentation

Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb Certificates of Analysis

TitleLot Number
475923

References

Přehled odkazů
Levey, A.I., et al. 1991. J. Neurosci. 11, 3218.
Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
Smith. D.B., et al. 1988. Gene 67, 31.
Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2007 RFH
SynonymsAnti-M1
ApplicationImmunoblotting (1:200-1:300, chemiluminescence)
Immunohistochemistry (see application references)
Immunoprecipitation (see application references)
Application Data
Detection of rat muscarinic M1 acetylcholine receptor by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the fusion protein antigen (lane 2). Primary antibody: Anti-Muscarinic M1 Acetylcholine Receptor (227-353) Rabbit pAb (Cat. No. 475923) (1:200). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody adsorbed against immobilized non-relevant GST fusion protein followed by immunoaffinity chromatography. Recognizes the ~120 kDa muscarinic M1 acetylcholine receptor protein. Supplied with a control protein consisting of recombinant M1-GST fusion protein.
HostRabbit
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 227-353 of the i3 intracellular loop of human M1 muscarinic acetylcholine receptor fused to GST
IsotypeIgG
Specieshuman, rat
Positive controlM1-GST fusion protein (supplied) or rat brain
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, 1% BSA, pH 7.4 and 60 µg lyophilized control protein.
Preservative≤0.1% sodium azide (antibody only)
CommentsThe epitope is specific for M1 and is not similar to any other known protein. To reduce non-specific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. For use as a positive control in immunoblotting, use 10 ng/lane of the M1-GST fusion protein (minigel). For use as a negative control, preincubate 3-5 µg of M1-GST fusion protein with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the antibody with 50 µl dH₂O to yield a stock concentration of 300 µg/ml. For long-term storage, aliquot the antibody and freeze (-20°); for short-term storage, refrigerate (4°C). Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the M1-GST fusion protein with 100 µl H₂O, aliquot, and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesLevey, A.I., et al. 1991. J. Neurosci. 11, 3218.
Levey, A.I., et al. 1990. FEBS Lett. 275, 65.
Smith. D.B., et al. 1988. Gene 67, 31.
Allard, W.J., et al. 1987. Nucleic Acids Res. 15, 10604.
Peralta, E.G., et al. 1987. EMBO J. 6, 3923.
Application referencesImmunohistochemistry, Immunoprecipitation Levey, A.I., et al. 1991. J. Neurosci. 11, 3218. Immunoprecipitation Levey, A.I., et al. 1990. FEBS Lett. 275, 65.