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ABE1076 Anti-Misu/NSun2 Antibody

Anti-Misu/NSun2 Antibody is an antibody against Misu/NSun2 for use in western blotting, ICC & IHC.

Anti-Misu/NSun2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: NSUN2, SAKI, TRM4 UniProt Number: Q08J23
ABE1076
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberABE1076
DescriptionAnti-Misu/NSun2 Antibody
Alternate Names
  • tRNA (cytosine(34)-C(5))-methyltransferase
  • Myc-induced SUN domain-containing protein
  • Misu
  • NOL1/NOP2/Sun domain family member 2
  • Substrate of AIM1/Aurora kinase B
  • tRNA (cytosine-5-)-methyltransferase
  • tRNA methyltransferase 4 homolog
  • hTrm4
Background InformationMisu/NSun2, also known as tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein (MISU), NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, or tRNA methyltransferase 4 homolog (hTRM4), and encoded by the gene NSUN2/SAKI/TRM4, is a RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Misu/NSun2 methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Functionally Misu/NSun2 acts downstream of Myc to regulate epidermal cell growth and proliferation, and Misu/NSun2-mediated cytosine-5 methylation of vault noncoding RNA helps to determine the processing of them into regulatory small RNAs, and Misu/NSun2 also plays a critical role in gene expression. Interestingly too, Misu/NSun2 is required for proper spindle assembly and chromosome segregation, which is independent of its methyltransferase activity. Misu/NSun2 is localized in the nucleus and nucleolus during rest but during cytokinesis it can be localized in the spindle. Mutations are associated with mental retardation.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Misu/NSun2 Antibody is an antibody against Misu/NSun2 for use in western blotting, ICC & IHC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human keratinocytes (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Misu/NSun2 in human epidermis tissue (Fry, M., and Watt, F. M. (2006). Curr Biol. 16(10):971-981.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Misu/NSun2.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NSUN2
  • SAKI
  • TRM4
Purification MethodUnpurified
UniProt Number
Molecular Weight~90 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Misu/NSun2 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE1076 04053252972911

Documentation

Anti-Misu/NSun2 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Misu/NSun2 Antibody Certificates of Analysis

TitleLot Number
Anti-Misu/NSun2 - Q2420922 Q2420922
Anti-Misu/NSun2 - 3284332 3284332
Anti-Misu/NSun2 -2695287 2695287

References

Reference overviewPub Med ID
The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.
Frye, Michaela and Watt, Fiona M
Curr. Biol., 16: 971-81 (2006)  2005

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