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IM1012 Anti-MTA3 (400-450) Rabbit pAb

This Anti-MTA3 (400-450) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of MTA3 (400-450).

Anti-MTA3 (400-450) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Metastasis-associated gene 3

IM1012
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~62 kDa MTA3 protein in MCF-7 cells.
Catalogue NumberIM1012
Brand Family Calbiochem®
SynonymsAnti-Metastasis-associated gene 3
Application Data
Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.

Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
References
ReferencesFujita, N., et. al. 2003. Cell 113, 207.
Kumar R. 2003. Cell 113, 142.
Simpson, A, et.al. 2001. Gene 273, 29.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlMCF7 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:500-1:5000)
Immunocytochemistry (1:300)
Immunoprecipitation (2-10 µg/mg lysate)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
IM1012 0

Documentation

Anti-MTA3 (400-450) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-MTA3 (400-450) Rabbit pAb Certificates of Analysis

TitleLot Number
IM1012

References

Přehled odkazů
Fujita, N., et. al. 2003. Cell 113, 207.
Kumar R. 2003. Cell 113, 142.
Simpson, A, et.al. 2001. Gene 273, 29.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
SynonymsAnti-Metastasis-associated gene 3
ApplicationImmunoblotting (1:500-1:5000)
Immunocytochemistry (1:300)
Immunoprecipitation (2-10 µg/mg lysate)
Application Data
Lane 1: Detection of human MTA3 by immunoblotting. Samples: Nuclear extract (25 µg) from MCF-7 cells. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:1000). Detection: chemiluminescence. Lanes 2 and 3: Detection of human MTA3 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (500 µg) (lane 2). Antibody for IP: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:100) and immunoprecipitation control (lane 3): nonspecific rabbit IgG (1:100). Immunoblotting conditions: same as lane 1. Detection: chemiluminescence.

Detection of human MTA3 by immunocytochemistry. Sample: Sk-Mel-28 cells fixed for 5 min with ice cold methanol and blocked with normal goat serum. Primary antibody: Anti-MTA3 (400-450) Rabbit pAb (Cat. No. IM1012) (1:300). Detection: fluorescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~62 kDa MTA3 protein.
BackgroundMTA1, MTA2, and MTA3 (Metastasis-associated gene 3, ACC# AAH53631) are members of a growing novel gene family associated with highly metastatic human carcinomas. MTA3 is expressed in all tissues as both 515 aa (MTA3) and 594 aa (MTA3L) proteins found in both the nucleus and the cytoplasmic compartments. It was identified as an Estrogen receptor (ER) regulated component of the Mi/NuRD histone deacetylase transcriptional repressor. In response to estrogen, MTA3 directly targets Snail, the master regulator of the epithelial to mesenchymal transition (EMT). Regulation of E-cadherin expression due to MTA3 and ER mediated signaling of Snail expression may be an important pathway in human breast tumors. Loss of ER results in decreased MTA3 expression and tamoxifen reduces expression of MTA3 in endometrial cells.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids within residues 400-450 of human MTA3
IsotypeIgG
Specieshuman
Positive controlMCF7 cells
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage.
Toxicity Highly Toxic
ReferencesFujita, N., et. al. 2003. Cell 113, 207.
Kumar R. 2003. Cell 113, 142.
Simpson, A, et.al. 2001. Gene 273, 29.