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MABF1413 Anti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody

This rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor™ 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).

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Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD74, Ii UniProt Number: P04441
MABF1413
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFCRvioletFluor™ 450Monoclonal Antibody
Description
Catalogue NumberMABF1413
DescriptionAnti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody
Alternate Names
  • H-2 class II histocompatibility antigen gamma chain
  • Ia antigen-associated invariant chain (Ii)
  • MHC class II-associated invariant chain
  • CD74
  • MHC class II (I-A/I-E)
Background InformationThe M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
Product Information
FormatvioletFluor™ 450
PresentationPurified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor™ 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneM5/114.15.2
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD74
  • Ii
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 μg
Global Trade Item Number
Katalogové číslo GTIN
MABF1413 04055977291407