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428017 Anti-Lamp1 Mouse mAb (LY1C6)

This Anti-Lamp1 Mouse mAb (LY1C6) is validated for use in Immunoblotting, Immunocytochemistry, Immunofluorescence, Immunoprecipitation for the detection of Lamp1.

Anti-Lamp1 Mouse mAb (LY1C6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Lysosome-Associated Membrane Protein 1

428017
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
RMMonoclonal Antibody

Products

Katalogové čísloBalení ks/bal.
428017-100UG Plastová ampulka 100 μg
Description
OverviewRecognizes the ~120 kDa Lamp1 protein.
Catalogue Number428017
Brand Family Calbiochem®
SynonymsAnti-Lysosome-Associated Membrane Protein 1
Application Data
Detection of hamster Lamp1 by immunocytochemistry. Sample: CHO K1 cells. Primary antibody: Anti-Lamp1 Mouse mAb (LY1C6) (Cat. No. 428017) (1:100). Detection: fluorescence.
References
ReferencesKannan, K. et al. 1996. Cell Immunol. 171, 10.
Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
Howe et al. 1988. PNAS 85, 7577.
Lewis et al. 1985. J. Cell Biol. 100, 1839.
Product Information
FormLiquid
FormulationIn PBS containing 50% glycerol, pH 7.2.
Positive controlCHO-K1 cells
Preservative≤0.09% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
Immunocytochemistry (1:100)
Immunofluorescence (see comments)
Immunoprecipitation (see comments)
Application CommentsThis antibody has also been reported to work for immunofluorescence and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrat liver lysosomal membranes
ImmunogenRat
CloneLY1C6
HostMouse
IsotypeIgG₁
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
428017-100UG 04055977210422

Documentation

Anti-Lamp1 Mouse mAb (LY1C6) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Lamp1 Mouse mAb (LY1C6) Certificates of Analysis

TitleLot Number
428017

References

Přehled odkazů
Kannan, K. et al. 1996. Cell Immunol. 171, 10.
Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
Howe et al. 1988. PNAS 85, 7577.
Lewis et al. 1985. J. Cell Biol. 100, 1839.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-June-2016 RB
SynonymsAnti-Lysosome-Associated Membrane Protein 1
ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
Immunocytochemistry (1:100)
Immunofluorescence (see comments)
Immunoprecipitation (see comments)
Application Data
Detection of hamster Lamp1 by immunocytochemistry. Sample: CHO K1 cells. Primary antibody: Anti-Lamp1 Mouse mAb (LY1C6) (Cat. No. 428017) (1:100). Detection: fluorescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~120 kDa Lamp1 protein.
BackgroundLyso-some-associated membrane protein (Lamp1), also known as lgpA, is a type 1 integral membrane protein that is transported from the trans-Golgi net-work to endosomes and then lysosomes. Upon cell activation, Lamp1 is transferred to the plasma membrane. Cell surface Lamp1 and Lamp2 promote adhesion of peripheral blood mononuclear cells (PBMC) to vascular endothelium and may be involved in the adhesion of PBMC to the site of inflammation.
HostMouse
Immunogen speciesRat
Immunogenrat liver lysosomal membranes
CloneLY1C6
IsotypeIgG₁
Speciesrat
Positive controlCHO-K1 cells
FormLiquid
FormulationIn PBS containing 50% glycerol, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.09% sodium azide
CommentsThis antibody has also been reported to work for immunofluorescence and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKannan, K. et al. 1996. Cell Immunol. 171, 10.
Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
Howe et al. 1988. PNAS 85, 7577.
Lewis et al. 1985. J. Cell Biol. 100, 1839.