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LP02 Anti-LDL Receptor Mouse mAb (15C8)

This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

Anti-LDL Receptor Mouse mAb (15C8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
LP02
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
B, HMMonoclonal Antibody

Products

Katalogové čísloBalení ks/bal.
LP02-100UG Plastová ampulka 100 μg
Description
OverviewRecognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
Catalogue NumberLP02
Brand Family Calbiochem®
Application Data
Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
References
Referencesvan Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
Russell, D.W., et al. 1984. Cell 37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
Goldstein, J.L., et al. 1979. Nature 279, 679.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlLiver sections, HT1080 cells, HepG2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.
Key Applications Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA (0.5-1 µg/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunocytochemistry (1-5 µg/ml, IF)
Immunoprecipitation (1-2 µg/sample)
Application CommentsWill displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena trypic digest of bovine low density lipoprotein receptor
ImmunogenBovine
Epitopefirst cysteine-rich ligand binding domain
Clone15C8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
LP02-100UG 04055977228076

Documentation

Anti-LDL Receptor Mouse mAb (15C8) Certificates of Analysis

TitleLot Number
LP02

References

Přehled odkazů
van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
Russell, D.W., et al. 1984. Cell 37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
Goldstein, J.L., et al. 1979. Nature 279, 679.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-June-2008 JSW
ApplicationELISA (0.5-1 µg/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunocytochemistry (1-5 µg/ml, IF)
Immunoprecipitation (1-2 µg/sample)
Application Data
Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
BackgroundThe low density lipoprotein receptor is responsible for mediating the uptake of LDL, a cholesterol carrying plasma lipoprotein. The receptor is a member of a class of cell surface proteins which are concentrated in clathrin coated pits and carry external ligands into cells by receptor-mediated endocytosis. The purified receptor is a glycoprotein with a molecular weight of 160,000. It is synthesized as a precursor of molecular weight 120,000 that is then glycosylated to the mature form by addition of N-linked and O-linked sugars in the Golgi apparatus. Unlike other hepatic lectins, the orientation of the LDL-R is such that the amino terminus is to the exterior of the cell. It has recently been determined that the LDL-R has sequence homology with the epidermal growth factor receptor precursor.
HostMouse
Immunogen speciesBovine
Immunogena trypic digest of bovine low density lipoprotein receptor
Epitopefirst cysteine-rich ligand binding domain
Clone15C8
IsotypeIgG2a
Speciesbovine, human, not rabbit
Positive controlLiver sections, HT1080 cells, HepG2 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsWill displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
Referencesvan Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
Russell, D.W., et al. 1984. Cell 37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
Goldstein, J.L., et al. 1979. Nature 279, 679.
Application referencesOriginal Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.