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ABC927 Anti-LDHB Antibody

Detect LDHb using this rabbit polyclonal Anti-LDHB Antibody, Cat. No. ABC927, validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Anti-LDHB Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: LDHB UniProt Number: P07195
ABC927
100 μg  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC927
DescriptionAnti-LDHB Antibody
Alternate Names
  • L-lactate dehydrogenase B chain (EC:1.1.1.27)
  • LDH-B
  • LDH heart subunit
  • LDH-H
  • Renal carcinoma antigen NY-REN-46
  • LDHB
Background InformationL-lactate dehydrogenase B chain (EC 1.1.1.27; UniProt P07195; also known as Epididymis secretory protein Li 281, Lactate dehydrogenase H chain, LDH-B, LDH-H, LDH heart subunit, Renal carcinoma antigen NY-REN-46) is encoded by the LDHB (also known as LDHBD, TRG-5, HEL-S-281) gene (Gene ID 3945) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. Lactate dehydrogenase (LDH) is a tetrameric enzyme composed of two different subunits, LDHA and LDHB, which can assemble into five different combinations (LDH1 is comprised of four LDHB subunits; LDH2 contains three LDHB and one LDHA subunit; LDH3 contains two LDHB and two LDHA subunits; LDH4 contains one LDHB and three LDHA subunits; LDH5 contains four LDHA subunits). LDHB is ubiquitously expressed and the predominant isoform in heart muscle, while LDHA is the predominant isoform in skeletal muscle and other highly glycolytic tissues. LDH catalyzes the reversible conversion of pyruvate into lactate. An upregulation of LDH therefore reduces the oxygen dependency and ensures an efficient anaerobic/glycolytic metabolism in tumor cells. Serum LDHB levels, for example, are reported to elevate in lung cancer patients and progressively increase with the clinical stage of non-small cell lung cancer (NSCLC).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect LDHb using this rabbit polyclonal Anti-LDHB Antibody, Cat. No. ABC927, validated for use in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected LDHB in normal human prostate tissue, as well as human cancer cells in Non-Hodgkin lymphoma, kidney, colon, and lung tumor tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human LDHB sequence from the C-terminal half.
EpitopeC-terminal half
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Horse, Rabbit, Canine, Rat, Pig, Bovine, and Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • LDHB
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~37 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human PANC-1 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected LDHB in 10 µg of human PANC-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABC927 04055977177022