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ABS97 Anti-KEAP1 Antibody

Anti-KEAP1 Antibody detects level of KEAP1 & has been published & validated for use in WB & IC.

Anti-KEAP1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: General Post-translation Modification Gene Symbol: KEAP1, INRF2, KLHL19 UniProt Number: Q14145
ABS97
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS97
DescriptionAnti-KEAP1 Antibody
Alternate Names
  • Cytosolic inhibitor of Nrf2
  • Kelch-like protein 19
  • INrf2
Background InformationAnti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2). Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor. Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes. The substrate adaptor function of Keap1 is inactivated during physiological stress. In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes. Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-KEAP1 Antibody detects level of KEAP1 & has been published & validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 2 µg/mL from a representative lot detected KEAP1 in A431 and HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the Kelch 3 region of human KEAP1.
EpitopeKelch 3 Region
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the Kelch 3 region of KEAP1.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Gene Symbol
  • KEAP1
  • INRF2
  • KLHL19
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.

SUBUNIT STRUCTURE: Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.
Molecular Weight~60-70 kDa observed.
A doublet may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected KEAP1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABS97 04053252591310

Documentation

Anti-KEAP1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-KEAP1 Antibody Certificates of Analysis

TitleLot Number
Anti-KEAP1 - 2345061 2345061
Anti-KEAP1 - 2370852 2370852
Anti-KEAP1 - 2199696 2199696
Anti-KEAP1 - 2279264 2279264
Anti-KEAP1 - 3205472 3205472
Anti-KEAP1 - 3313126 3313126
Anti-KEAP1 - 3761217 3761217
Anti-KEAP1 - 3840877 3840877
Anti-KEAP1 - 3862692 3862692
Anti-KEAP1 - 4097773 4097773
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References

Reference overviewPub Med ID
Propofol activation of the Nrf2 pathway is associated with amelioration of acute lung injury in a rat liver transplantation model.
Yao, W; Luo, G; Zhu, G; Chi, X; Zhang, A; Xia, Z; Hei, Z
Oxidative medicine and cellular longevity  2014  258567  2014

Zobrazit abstrakt 		24669282 24669282

Brochure

Title
New Products: Volume 3, 2012