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MAB2247 Anti-Integrin β1 Antibody, clone 12G10

MAB2247
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, IP, FUNC, CULT, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2247
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β1 Antibody, clone 12G10
Alternate Names
  • Integrin beta-1
  • CD29
  • Glycoprotein Iia
  • GPIIA
  • Integrin VLA-4 subunit beta
  • Very late activation protein
  • beta polypeptide
Background InformationIntegrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
Product Information
FormatPurified
Control
  • Tonsil, human skin tissue
PresentationPurified immunoglobulin. Liquid in phosphate buffer saline, with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Affects Function
  • Cell Culture
  • Western Blotting
Application NotesFlow cytometry: 1:25-1:50

ELISA : 10 μg/mL

Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.

Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
Clone12G10
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityMAB2247 recognizes the human beta1 integrin. This antibody has been shown to recognize a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. This antibody also enhances alpha 5 - beta 1 fibronectin interactions. MAB2247 does not cross-react with mouse or rat samples.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGB1
  • FNRB
  • MDF2
  • MSK12
  • VLA-BETA
  • VLAB
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight88 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2–8°C in undiluted aliquots for up one month. For long term storage maintain at -20°C for up to 12 months.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
MAB2247 04053252661099