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ABS2090-25UL Anti-ICDH

Anti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.

Research Category: Signaling Gene Symbol: ICDH UniProt Number: No verified Uniprot, PUBMED 25977442
ABS2090-25UL
25 µL  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
E. coliWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABS2090-25UL
DescriptionAnti-ICDH
Alternate Names
  • Isocitrate dehydrogenase
  • ICDH (NADP)
  • IDH
Background InformationIsocitrate dehydrogenase [NADP] (also known as IDH, ICDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In eukaryotes there are at least three isozymes of IDH: two are located in the mitochondrial matrix (one NAD+-dependent, the other NADP+-dependent), while the third one (also NADP+-dependent) is cytoplasmic. In E.coli the activity of a NADP+-dependent ICDH is controlled by the phosphorylation of a serine residue and the phosphorylated form of ICDH is completely inactivated. It has been reported that if Ser113 is replaced by alanine, cysteine, threonine, or tyrosine there is some loss of enzymatic activity. However, substitution by aspartic acid completely abolishes its activity. ICDH contains three domains: a large alpha + beta domain, a small alpha/beta domain and an alpha/beta clasp-like domain involving both subunits. (Ref.: Hurley, JH et al (1989). Proc. Natl. Acad. Sci. USA. 86 (22); 8635-8639).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-ICDH, Cat. No. ABS2090, is a rabbit polyclonal antibody that detects Isocitrate dehydrogenase [NADP]. It has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected ICDH in 20 UL Legionella cell lysate.
Western Blotting Analysis: A representative lot detected ICDH in E. Coli cell lysate (Xu, L., et. al. (2010). PLoS Pathog. 6(3):e1000822; Lu, C., et. al. (2016). Sci Signal. 9(412):ra11).
Biological Information
ImmunogenHis-tagged full length recombinant isocitrate dehydrogenase from Legionella pneumophila, strain Lp02.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects isocitrate dehydrogenase in E. coli and Legionella pneumophila.
IsotypeIgG
Species Reactivity
  • E. coli
Species Reactivity NoteE. Coli, Legionella pneumophila.
Antibody TypePolyclonal Antibody
Gene Symbol
  • ICDH
Purification MethodUnpurified
UniProt Number
Molecular Weight45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in E.Coli cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected ICDH in 20 UL E.Coli cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABS2090-25UL 04054839505577

Documentation