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CP53 Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Inter-Cellular Adhesion Molecule, Anti-CD54

CP53
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Přehled

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Katalogové čísloBalení ks/bal.
CP53-100UG Plastová ampulka 100 μg
Přidat k oblíbeným položkám
Description
OverviewRecognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.
Catalogue NumberCP53
Brand Family Calbiochem®
SynonymsAnti-Inter-Cellular Adhesion Molecule, Anti-CD54
References
ReferencesTakami, S., et al. 1998. Circ. 97, 721.
Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
DeGraba, T.J. 1997. Neurology 49, S15.
Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
Fawcett, J., et al. 1992. Nature 360, 481.
Vazeux, R. et al. 1992. Nature 360, 485.
Boyd, A.W., et al. 1989. Blood 73, 1896.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlEndothelial cells or tonsil tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Boyd, A.W., et al. 1988. PNAS 85, 3095.
Key Applications Cell Inhibition Assays
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Paraffin Sections
Application NotesCell Inhibition Assays (see comments)
Flow Cytometry (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunofluorescence (see comments)
Immunoprecipitation (10 µl/mg protein lysate)
Paraffin Sections (not recommended)
Application CommentsThis antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenRaji cells
ImmunogenHuman
CloneW-CAM-1
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Global Trade Item Number
Katalogové číslo GTIN
CP53-100UG 04055977221008