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CA1023 Anti-Hsp90α Mouse mAb (EMD-17D7)

CA1023
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Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Katalogové čísloBalení ks/bal.
CA1023-50UG Plastová ampulka 50 μg
Description
OverviewRecognizes the ~90 kDa Hsp90α protein in HeLa cells. Does not recognize Hsp90β.
Catalogue NumberCA1023
Brand Family Calbiochem®
SynonymsAnti-Heat Shock Protein 90α
Application Data
Detection of human Hsp90α by immunoblotting. Samples: Whole cell lysate (50 μg) from HeLa cells (lane A), Hsp90β, His•Tag®, Human, Recombinant (Cat. No. 385903) (100 ng, lane B), and Hsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901) (100 ng, lane C). Primary antibody: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (1 μg/ml). Detection: chemiluminescence.

Detection of human Hsp90α by immunoprecipitation and immunoblotting. Sample: Whole cell lysate (700 μg) from H-460 cells. Antibody for IP: Anti-Hsp90α Mouse mAb (EMD-17D7) (Cat. No. CA1023) (5 μg/700 μg protein). Conditions for immunoblotting: Sample: 10 μl of 50 μl Hsp90α IP (above). Primary antibody: Anti-Hsp90α Rabbit pAb (Cat. No. CA1016) (1:5000). Detection: chemiluminescence.
References
ReferencesScheibel, T., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1297.
Nemoto, T., et al. 1997 J. Biol. Chem. 272, 26179.
Nemoto, T., et al. 1998. Biochem. J. 330, 989.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlH460 and HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (see comments)
Immunoblotting (1 µg/ml)
Immunoprecipitation (5 µg/0.7 mg protein)
Application CommentsDetects native and recombinant Hsp90α. Does not recognize Hsp90β. This antibody will also work for ELISA, but concentration is assay dependent. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenHsp90α, His•Tag®, Human, Recombinant (Cat. No. 385901)
ImmunogenHuman
CloneEMD-17D7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Katalogové číslo GTIN
CA1023-50UG 04055977220780