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382160 Anti-Histone H4, Acetylated (2-19) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
382160
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
H, TRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~10 kDa acetylated histone H4 protein in Tetrahymena and sodium butyrate-treated HeLa cells.
Catalogue Number382160
Brand Family Calbiochem®
Application Data
Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Positive controlTetrahymena and sodium butyrate-treated HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (0.5-2 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (5µg/500 µg lysate)
Application CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
Biological Information
Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
ImmunogenTetrahymena
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Tetrahymena
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
382160 0

Documentation

Anti-Histone H4, Acetylated (2-19) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Histone H4, Acetylated (2-19) Rabbit pAb Certificates of Analysis

TitleLot Number
382160

References

Přehled odkazů
Braunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
ApplicationImmunoblotting (0.5-2 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (5µg/500 µg lysate)
Application Data
Detection of human acetylated histone H4 by immunoblotting. Samples: Acid-extracted proteins from HeLa cells treated with sodium butyrate at antibody concentrations of 0.5 µg/ml (lane 1) and 2 µg/ml (lane 2). Primary antibody: Anti-Histone H4, Acetylated (2-19) Rabbit pAb (Cat. No. 382160) (2 µg/ml). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~10 kDa acetylated histone H4 protein.
BackgroundThe acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
HostRabbit
Immunogen speciesTetrahymena
Immunogena tetra-acetylated synthetic peptide [AGGAcKGGAcKGMGAcKVGAAcKRHS(C)] corresponding to amino acids 2-19 of Tetrahymena histone H4, conjugated to KLH
IsotypeIgG
SpeciesTetrahymena, human
Positive controlTetrahymena and sodium butyrate-treated HeLa cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe antibody detected acetylated histone H4 in acid-extracted proteins from HeLa cells. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with histone H2B from HeLa cells. May cross-react with other acetylated proteins. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H4. For immunocytochemistry, human A431 cells were treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h and fixed with ice-cold 1% paraformaldehyde in PBS. Variables associated with assay conditions will dictate the optimal dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
Braunstein, M., et al. 1993. Genes Devel. 7, 592.
Perry, C.A., et al. 1993. Biochemistry 32, 13605.
Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.