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PC544 Anti-Histone Deacetylase 1 (467-482) Rabbit pAb

Anti-Histone Deacetylase 1 (467-482), rabbit polyclonal, recognizes the ~62 kDa HDAC1 in HeLa, HepG2, DU145, PC3, and MCF-7 cells. It is validated for use in Western blotting and immunofluorescence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HDAC1

PC544
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
Ca, Ht, H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~62 kDa HDAC1 protein in HeLa, HepG2, DU145, PC3, and MCF-7 cells.
Catalogue NumberPC544
Brand Family Calbiochem®
SynonymsAnti-HDAC1
Application Data
Detection of human histone deacetylase 1 by immunocytochemistry. Sample: HeLa cells. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (2 µg/ml). Detection: fluorescence.

Detection of human Histone Deacetylease 1 by immunoblotting. Sample: Whole cell lysate from HeLa cell. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (0.5 µg/ml). Detection: chemiluminescence.
References
ReferencesCress, W.D. and Seto, E. 2000. J Cell Physiol. 184, 1.
Juan, L.J., et al. 2000. J Biol Chem. 275, 20436.
Taunton, J., et al. 1996. Science 272, 408.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Positive controlHeLa, HepG2, Du145, PC-3, or MCF7 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting (0.5 µg/ml)
Immunocytochemistry (2 µg/ml)
Immunofluorescence (1 µg/ml)
Immunohistochemistry (0.2 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 467-482 of human histone deacetylase 1
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Canine
  • Hamster
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC544 0

Documentation

Anti-Histone Deacetylase 1 (467-482) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Histone Deacetylase 1 (467-482) Rabbit pAb Certificates of Analysis

TitleLot Number
PC544

References

Přehled odkazů
Cress, W.D. and Seto, E. 2000. J Cell Physiol. 184, 1.
Juan, L.J., et al. 2000. J Biol Chem. 275, 20436.
Taunton, J., et al. 1996. Science 272, 408.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2007 RFH
SynonymsAnti-HDAC1
ApplicationImmunoblotting (0.5 µg/ml)
Immunocytochemistry (2 µg/ml)
Immunofluorescence (1 µg/ml)
Immunohistochemistry (0.2 µg/ml)
Application Data
Detection of human histone deacetylase 1 by immunocytochemistry. Sample: HeLa cells. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (2 µg/ml). Detection: fluorescence.

Detection of human Histone Deacetylease 1 by immunoblotting. Sample: Whole cell lysate from HeLa cell. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~62 kDa HDAC1 protein.
BackgroundThe regulation of eukaryotic gene expression is marked by post-translational modification of nucleosomal histones, a key event that converts regions of chromosomes into transcriptionally active or inactive chromatin. A single nucleosomal core particle contains an octamer of histone proteins H2A, H2B, H3 and H4. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery. There are at least eight cloned human histone deacetylases. Studies have pointed to the possible involvement of histone deacetylases in human cancer. Histone Deacetylase 1 has been shown to interact directly with transcription factors and to deacetylate histone proteins H3 and H4. Studies have also shown that mammalian histone deacetylase (HDAC)-1, -2, and -3 are all capable of down-regulating p53 function. p53, the most commonly mutated gene in cancer cells, directs cell cycle arrest or induces programmed cell death (apoptosis) in response to stress. HDACs are classified by their sequence homology to the yeast HDACs. Class I proteins are related to Rpd3 and members of class II resemble HdaIp.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 467-482 of human histone deacetylase 1
IsotypeIgG
Speciescanine, hamster, human, mouse, rat
Positive controlHeLa, HepG2, Du145, PC-3, or MCF7 cells
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCress, W.D. and Seto, E. 2000. J Cell Physiol. 184, 1.
Juan, L.J., et al. 2000. J Biol Chem. 275, 20436.
Taunton, J., et al. 1996. Science 272, 408.