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ABT1385 Anti-Glypican-1

Anti-Glypican-1 Antibody, Cat. No. ABT1385 is a rabbit polyclonal antibody that detects Glypican-1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Anti-Glypican-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Gpc1 UniProt Number: P35053
ABT1385
100 µL  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
RICC, IHC, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberABT1385
DescriptionAnti-Glypican-1
Alternate Names
  • HSPG M12
  • GPC1
Background InformationGlypican-1 (UniProt: P35053; also known as GPC1, HSPG M12) is encoded by the Gpc1 gene (GeneID: 58920) in rat. Glypican-1 is attaches to the plasma membrane via glycosylphosphatidylinositol (GPI) anchors. It is a cell surface proteoglycan that bears heparan sulfate and may act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Glypican-1 is required for proper skeletal muscle differentiation. It sequesters FGF2 in lipid rafts and blocks its binding to FGF receptors. Glypican-1 is specifically enriched on cancer-cell-derived exosomes and levels of glypican-1 positive exosomes correlate with tumor burden and the survival of pre- and post-surgical patients. Glypican-1 expression, both mRNA and protein, is upregulated in human pancreatic cancers cells and adjacent fibroblasts. (Ref.: Melo, SA et al. 2015. Nature. 523(7559):177-82; Kleeff, J et al. 1998. J. Clin. Invest. 102(9), 1662 1673).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Glypican-1 Antibody, Cat. No. ABT1385 is a rabbit polyclonal antibody that detects Glypican-1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Glypican-1 in rat brain homogenates and rat C6 glioma cells treated with heparitinase (Liang, Y., et. al. (1997). J Cell Biol. 139(4):851-64).

Immunocytochemistry Analysis: A representative lot detected Glypican-1 in rat C6 glioma cells (Liang, Y., et. al. (1997). J Cell Biol. 139(4):851-64).

Immunohistochemistry Analysis: A representative lot detected Glypican-1 in rat spinal cord and brain (Liang, Y., et. al. (1997). J Cell Biol. 139(4):851-64).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to the first 423 amino acids of rat Glypican-1.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Glypican-1 in Rat C6 glioma cells.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gpc1
Purification MethodUnpurified
UniProt Number
Molecular Weight61.73 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in Rat C6 glioma cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Glypican-1 in Rat C6 glioma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABT1385 04054839240539