Pokud jste položku nepřidali do nákupního košíku nebo do oblíbených, vaše konfigurace se při zavření neuloží.
Chcete-li zavřít nástroj MILLIPLEX® MAP, klikněte na OK. Pokud se chcete vrátit ke svým výběrům, klikněte na Zrušit.
Vyberte přizpůsobitelné panely a soupravy smíchané předem – NEBO - soupravy buněčné signalizace MAPmates™
Navrhněte si a oceňte své soupravy MILLIPLEX® MAP.
Přizpůsobitelné panely a soupravy smíchané předem
Naše široké portfolio se skládá z multiplexních panelů, které vám umožňují vybrat v rámci panelu analyty přesně podle vašich představ. Na zvláštní kartě si můžete zvolit formát předem smíchaných cytokinů, nebo soupravu single plex.
Soupravy buněčné signalizace a MAPmates™
Vyberte pevné soupravy, které vám umožní prozkoumat celé cesty nebo procesy. Nebo si sestavte své vlastní soupravy ze single plex MAPmates™, při dodržení uvedených pokynů.
Následující MAPmates™ by se neměly spolu mísit: -MAPmates™ vyžadující jiný testovací pufr -Fosfo-specifické a celkové páry MAPmate™, např. celkové GSK3β a GSK3β (Ser 9) -PanTyr a MAPmates™ specifické pro lokalitu, např. receptor fosfo-EGF a fosfo-STAT1 (Tyr701) -Více než 1 fosfo-MAPmate™ pro jediný cíl (Akt, STAT3) -GAPDH a β-Tubulin nelze mísit se soupravami nebo MAPmates™ obsahujícími panTyr
.
Katalogové číslo
Popis objednávky
ks/bal.
Seznam
Položka byla přidána do oblíbených.
Vyberte druh, typ panelu, soupravu nebo typ vzorku
Chcete-li začít s návrhem vaší soupravy MILLIPLEX® MAP, vyberte příslušný druh, typ panelu nebo soupravu.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Položka byla přidána do oblíbených.
Druh
Typ panelu
Vybraná souprava
ks
Katalogové číslo
Popis objednávky
ks/bal.
Cena podle ceníku
96-Well Plate
ks
Katalogové číslo
Popis objednávky
ks/bal.
Cena podle ceníku
Přidat další reagencie (Pro použití s MAPmates jsou vyžadovány pufr a detekční souprava)
ks
Katalogové číslo
Popis objednávky
ks/bal.
Cena podle ceníku
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Možnost úspory místa Zákazníci, kteří si pořizují různé soupravy, se mohou rozhodnout pro úsporu místa tím, že nevyžadují balení soupravy a zboží si nechají dodat ve formě multiplexních komponent testů v plastových sáčcích, které se pak kompaktněji skladují.
Položka byla přidána do oblíbených.
Produkt byl přidán do vašeho nákupního košíku
Nyní můžete upravit další soupravu, vybrat předem smíchanou soupravu, odhlásit se nebo zavřít objednací nástroj.
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More
IF03L
Sigma-AldrichAnti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5)
Anti-Glial Fibrillary Acidic Protein, mouse monoclonal, clone G-A-5, recognizes GFAP in astrocytes, astrocytomas, glial cells & gliomas. It is validated for use in WB, IF, IP, IHC (frozen & paraffin).
More>>Anti-Glial Fibrillary Acidic Protein, mouse monoclonal, clone G-A-5, recognizes GFAP in astrocytes, astrocytomas, glial cells & gliomas. It is validated for use in WB, IF, IP, IHC (frozen & paraffin). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes GFAP in astrocytes, astrocytomas, glial cells, gliomas, and other glial cell-derived tumors.
Catalogue Number
IF03L
Brand Family
Calbiochem®
Synonyms
Anti-GFAP
References
References
Droese, M., et al. 1984. Acta. Cytol.28, 368. Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191. Geisler, N. and Weber, K. 1983. EMBO J.11, 2059. Osborn, M. and Weber, K. 1983. Lab. Invest.48, 372. Altmannsberger, M., et al. 1982. Lab. Invest.46, 520. Lazarides, E. 1982. Ann. Rev. Biochem.51, 219. Osborn, M. and Weber, K. 1982. Cell31, 303.
Immunoblotting, Paraffin Sections
Sennlaub, F., et al. 2003. Circulation108, 198.
Original Clone
Debus, E., et al. 1983. Differentiation25, 193.
Immunofluorescence
Debus, E., et al. 1983. Differentiation25, 193.
Immunoblotting (see application references) Paraffin Sections (see application references) Frozen Sections (see comments) Immunofluorescence (see application references) Immunoprecipitation (see comments)
Application Comments
Stains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
GFAP
Immunogen
Porcine
Clone
G-A-5
Host
Mouse
Isotype
IgG₁
Species Reactivity
Chicken
Human
Porcine
Rat
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo
GTIN
IF03L-100UG
04055977228120
Documentation
Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5) Certificates of Analysis
Title
Lot Number
IF03L
References
Přehled odkazů
Droese, M., et al. 1984. Acta. Cytol.28, 368. Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191. Geisler, N. and Weber, K. 1983. EMBO J.11, 2059. Osborn, M. and Weber, K. 1983. Lab. Invest.48, 372. Altmannsberger, M., et al. 1982. Lab. Invest.46, 520. Lazarides, E. 1982. Ann. Rev. Biochem.51, 219. Osborn, M. and Weber, K. 1982. Cell31, 303.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-August-2007 RFH
Synonyms
Anti-GFAP
Application
Immunoblotting (see application references) Paraffin Sections (see application references) Frozen Sections (see comments) Immunofluorescence (see application references) Immunoprecipitation (see comments)
Description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the glial fibrillary acidic protein (GFAP).
Background
Intermediate (10 nm) filaments are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (glial fibrillary acidic protein or GFA or GFAP), skeletal, visceral, and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). GFAP and vimentin are coexpressed in at least some astrocytes. Anti-GFAP (Ab-1) identifies the GFAP subclass of intermediate filaments in human tissues.
Host
Mouse
Immunogen species
Porcine
Immunogen
GFAP
Clone
G-A-5
Isotype
IgG₁
Species
chicken, human, porcine, rat
Positive control
Glial cells (such as U343/333MG)
Negative control
Epithelial cells
Form
Lyophilized
Formulation
Lyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
Preservative
None
Comments
Stains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
Toxicity
Standard Handling
References
Droese, M., et al. 1984. Acta. Cytol.28, 368. Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191. Geisler, N. and Weber, K. 1983. EMBO J.11, 2059. Osborn, M. and Weber, K. 1983. Lab. Invest.48, 372. Altmannsberger, M., et al. 1982. Lab. Invest.46, 520. Lazarides, E. 1982. Ann. Rev. Biochem.51, 219. Osborn, M. and Weber, K. 1982. Cell31, 303.
Application references
Immunoblotting, Paraffin Sections
Sennlaub, F., et al. 2003. Circulation108, 198.
Original Clone
Debus, E., et al. 1983. Differentiation25, 193.
Immunofluorescence
Debus, E., et al. 1983. Differentiation25, 193.