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ABE1934 Anti-Gcn5 Antibody

Anti-Gcn5 Antibody is an antibody against Gcn5 for use in Immunoprecipitation, Western Blotting, ChIP-seq, Chromatin Immunoprecipitation (ChIP).

Anti-Gcn5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: KAT2A, GCN5, GCN5L2, HGCN5 UniProt Number: Q92830
ABE1934
100 µL  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIP, WB, ChIP-seq, ChIPRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE1934
DescriptionAnti-Gcn5 Antibody
Alternate Names
  • Histone acetyltransferase KAT2A
  • General control of amino acid synthesis protein 5-like 2
  • hGCN5
  • Histone acetyltransferase GCN5
  • HsGCN5
  • Lysine acetyltransferase 2A
  • STAF97
Background InformationHistone acetyltransferase KAT2A (EC 2.3.1.48; UniProt Q92830; also known as General control of amino acid synthesis protein 5-like 2, hGCN5, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97) is encoded by the KAT2A (also known as GCN5, GCN5L2, HGCN5) gene (Gene ID 2648) in human. Histone acetyltransferases (HATs) are transcription co-activators recruited by transcriptional activators to target gene promoters, where they enhance the transcription activity by acetylating lysine residues within the N-terminal regions of histones. KAT2A/KAT2B, MYST (MOZ, Ybf2 (Sas3), Sas2, and Tip60), TAFII250, CREBBP/EP300, and SRC represent the five HAT families. In addtion to functioning as transcription co-activators, KAT2A (GCN5) and KAT2B (PCAF) are also reported to repress IFN‐β production and innate antiviral immunity by inhibiting the innate immune signaling kinase TBK1 in the cytoplasm in a HAT‐independent and non‐transcriptional manner.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Gcn5 Antibody is an antibody against Gcn5 for use in Immunoprecipitation, Western Blotting, ChIP-seq, Chromatin Immunoprecipitation (ChIP).
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • ChIP-seq
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blotting Analysis: 1.2 µg/mL from a representative lot detected Gcn5 immunoprecipited from HeLa nuclear extract.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immunoprecipitated KAT2A/GCN5-associated DNA fragments by ChIP using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et al. (2014). Nature. 515(7527):371-375).
ChIP-seq Analysis: A representative lot was employed to generate Gcn5 genome-wide binding profiles by ChIP-seq analysis using chromatin preparations from murine CH12 and human GM12878 lymphoblasts (Cheng, Y., et al. (2014). Nature. 515(7527):371-375).
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to the N-terminal sequence of human Gcn5.
EpitopeN-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody was raised against human KAT2A isoform GCN5-L N-terminal sequence that is 95% conserved in murine and rat Kat2a/Gcn5, but missing in human spliced isoform GCN5-S (UniProt Q92830-2).
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • KAT2A
  • GCN5
  • GCN5L2
  • HGCN5
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~100 kDa observed. 93.93 kDa (human GCN5-L; UniProt Q92830-1) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunoprecipitation in HeLa nuclear extract.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipited Gcn5 from 500 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE1934 04055977335194