Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS2239-25UL Anti-GPAT4

Anti-GPAT4, Cat. No. ABS2239, is a rabbit polyclonal antibody that detects GPAT and is tested for use in Immunoprecipitation and Western Blotting.

ABS2239-25UL
25 µL  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Catalogue NumberABS2239-25UL
DescriptionAnti-GPAT4
Alternate Names
  • Glycerol-3-phosphate acyltransferase 4
  • EC:2.3.1.15
  • 1-acylglycerol-3-phosphate O-acyltransferase 6
  • 1-AGP acyltransferase 6
  • 1-AGPAT 6
  • Acyl-CoA:glycerol-3-phosphate acyltransferase 4
  • Lysophosphatidic acid acyltransferase zeta
  • LPAAT-zeta
  • Testis spermatogenesis apoptosis-related protein 7
  • TSARG7
Background InformationGlycerol-3-phosphate acyltransferase 4 (UniProt: Q86UL3; also known as EC:2.3.1.15, 1-acylglycerol-3-phosphate O-acyltransferase 6, 1-AGP acyltransferase 6; 1-AGPAT 6, Acyl-CoA:glycerol-3-phosphate acyltransferase 4, Lysophosphatidic acid acyltransferase zeta, LPAAT-zeta, Testis spermatogenesis apoptosis-related protein 7, TSARG7) is encoded by the GPAT4 (also known as AGPAT6, TSARG7, UNQ551/PRO1108) gene (Gene ID: 137954) in human. GPAT4 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase family that plays a role in triglyceride biosynthesis in several tissues. It is a rate-limiting enzyme that converts glycerol-3-phosphate (G3P) to 1-acyl-sn-glycerol-3-phosphate by incorporating an acyl moiety at the sn-1 position of the glycerol backbone. It is shown to be active against both saturated and unsaturated long-chain fatty acyl-CoAs. Its HXXXXD motif (aa 248-253) is reported to be essential for its acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. By increasing triglyceride synthesis, it protects cells against lipotoxicity induced by high levels of saturated fatty acids. (Ref.: Piccolis, M., et al. (2019). Mol. Cell. 74(1); 32-44; Chen, YQ., et al. (2008). J. Biol. Chem. 283(15); 10048-10057).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-GPAT4, Cat. No. ABS2239, is a rabbit polyclonal antibody that detects GPAT and is tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesTested applications

Immunoprecipitation Analysis: A representative lot Immunoprecipitated Human GPAT4 protein in Immunoprecipitation applications (Zhu, X.G., et al. (2019). Mol Cell. 74(1):45-58).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 25 amino acids from the N-termina half of human Glycerol-3-phosphate acyltransferase 4 (GPAT4).
EpitopeN-terminal half
Concentration0.35 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Glycerol-3-phosphate acyltransferase 4 (GPAT4). It targets an epitope within 25 amino acids from the N-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Porcine, Rat, Feline, Monkey, Mouse, Horse, Bovine, Canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GPAT4
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~46 kDa observed; 52.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Human GPAT4 protein in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABS2239-25UL 04065269135203