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07-1401 Anti-GLUT-1 Antibody, CT

07-1401
50 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information
Replacement Information07-1401 is a recommended replacement for AB1340

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1401
ReplacesAB1340
DescriptionAnti-GLUT-1 Antibody, CT
Alternate Names
  • Glucose Transporter type 1
  • Solute carrier family 2, facilitated glucose transporter, member 1
Background InformationSolute carrier family 2, facilitated glucose transporter member 1 (UniProt: P11166; also known as Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter) is encoded by the SLC2A1 (also known as GLUT1) gene (Gene ID: 6513) in human. Glucose transporters are a family of integral membrane proteins that facilitative glucose uptake by cells. Seven different glucose transport proteins have been described that are designated as GLUT-1 to 7. GLUT-1 is a highly conserved; ubiquitously distributed, multi-pass membrane protein that is responsible for constitutive glucose uptake. It displays very broad substrate specificity and can transport a wide range of aldoses including both pentoses and hexoses. It is the predominant glucose transporter in embryonic and fetal tissues. In many organs, GLUT-1 is concentrated in endothelial cells of blood-tissue barriers. Hence, it has a specialized role to shuttle glucose between blood and organs that have limited access to small solutes via passive diffusion. It is also abundant in the mammalian erythrocyte membrane where it can rapidly equilibrate glucose between the cytoplasm of the erythrocyte and the blood plasma. GLUT-1 levels are reported to be frequently upregulated during tumorigenesis. Mutations in SLC2A1 gene are linked to GLUT-1 deficiency syndrome 1 and 2 that are characterized by infantile-onset epileptic encephalopathy, delayed development, microcephaly, and motor incoordination, and paroxysmal exercise-induced dyskinesia.
Product Information
FormatAffinity Purified
Control
  • Jurkat Cell Lysate
PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GLUT-1, CT, Cat. No. 07-1401, is a rabbit polyclonal antibody that detects GLUT-1, CT and is tested for use in Western Blotting, Immunohistochemistry (Paraffin), and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
Application NotesTested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected GLUT-1 in A-431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GLUT-1 in human pancreas, human placenta, and human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal of Human Glucose transporter-1 (GLUT-1).
EpitopeC-Terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Glucose transporter type 1 (GLUT-1). It targets an epitope within 12 amino acids from the C-terminal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq].

Gene Symbol
  • GLUT
  • GLUT-1
  • GLUT1
  • MGC141895
  • MGC141896
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Melanosome. Note=Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: Expressed at variable levels in many human tissues.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: Defects in SLC2A1 are the cause of autosomal dominant GLUT1 deficiency syndrome [MIM:606777]. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily [view classification].

Molecular Weight~54 kDa observed; 54.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human umbilical vein endothelial cells (HUVEC) lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GLUT-1 in Human umbilical vein endothelial cell (HUVEC) lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size50 µg
Global Trade Item Number
Katalogové číslo GTIN
07-1401 08436037126307