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MABN794 Anti-GLAST Antibody, clone 8C11.1

Anti-GLAST Antibody, clone 8C11.1 is an antibody against GLAST for use in Western Blotting, Immunohistochemistry .

Anti-GLAST Antibody, clone 8C11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SLC1A3, EAAT1, GLAST, GLAST1 UniProt Number: P43003
MABN794
100 μg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN794
DescriptionAnti-GLAST Antibody, clone 8C11.1
Alternate Names
  • Excitatory amino acid transporter 1
  • Sodium-dependent glutamate/aspartate transporter 1
  • GLAST-1
  • Solute carrier family 1 member 3
  • GLAST
Background InformationExcitatory amino acid transporter 1 (UniProt P43003; also known as GLAST-1, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 member 3) is encoded by the SLC1A3 (also known as EA6, EAAT1, GLAST, GLAST1) gene in human (Entrez Gene ID 6507). GLAST-1 is a multitransmembrane protein that functions as a high affinity glutamate transporter and mediates the termination of postsynaptic action of glutamate in central nervous system by rapidly removing released glutamate from the synaptic cleft. GLAST-1 also transports L- and D-aspartate. GLAST gene mutations are linked to episodic ataxia type 6 (EA6), a disorder characterized by seizures, migraine, and alternating hemiplegia. There are 5 known spliced variants. Isoform 2 has no functional glutamate uptake activity and exerts a dominant negative effect against isoform 1.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1λ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GLAST Antibody, clone 8C11.1 is an antibody against GLAST for use in Western Blotting, Immunohistochemistry .
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLAST in human cerebellum and rat cerebellum tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the extracellular domain of human GLAST.
EpitopeExtracellular domain
Clone8C11.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8C11.1 epitope lies within the second extracellular segement of GLAST isoforms 1 & 2. The epitope region is also present in isoform 4, but not in isforms 3 & 5.
IsotypeIgG1λ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC1A3
  • EAAT1
  • GLAST
  • GLAST1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GLAST in 200 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABN794 04055977166644

Documentation

Anti-GLAST Antibody, clone 8C11.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-GLAST Antibody, clone 8C11.1 Certificates of Analysis

TitleLot Number
Anti-GLAST, clone 8C11.1 - 3167609 3167609
Anti-GLAST, clone 8C11.1 - 3250991 3250991
Anti-GLAST, clone 8C11.1 - 3389915 3389915
Anti-GLAST, clone 8C11.1 - 3866884 3866884
Anti-GLAST, clone 8C11.1 - 3944522 3944522
Anti-GLAST, clone 8C11.1 - 4163455 4163455
Anti-GLAST, clone 8C11.1 -2710635 2710635
Anti-GLAST, clone 8C11.1 -2764892 2764892
Anti-GLAST, clone 8C11.1 -2814887 2814887
Anti-GLAST, clone 8C11.1 -Q2569068 Q2569068
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Technical Info

Title
White Paper: Further considerations of antibody validation and usage.