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PC213L Anti-GABA Rabbit pAb

Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.

Anti-GABA Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Gamma Amino-Butyric Acid

PC213L
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
RRbPolyclonal Antibody

Products

Katalogové čísloBalení ks/bal.
PC213L-100UL Plastová ampulka 100 ul
Description
OverviewRecognizes GABA in rat thalamus and cerebellum.
Catalogue NumberPC213L
Brand Family Calbiochem®
SynonymsAnti-Gamma Amino-Butyric Acid
Application Data
Detection of rat GABA by immunohistochemistry. Sample: Rat thalamus and cerebellum. Primary antibody: Anti-GABA Rabbit pAb (Cat. No. PC213L) (1:20,000). Detection: fluorescence.
References
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat thalamus or cerebellum
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Application NotesFrozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
Application CommentsAntibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenGABA
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC213L-100UL 04055977224665

Documentation

Anti-GABA Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-GABA Rabbit pAb Certificates of Analysis

TitleLot Number
PC213L

References

Přehled odkazů
Sudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
SynonymsAnti-Gamma Amino-Butyric Acid
ApplicationFrozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)
Application Data
Detection of rat GABA by immunohistochemistry. Sample: Rat thalamus and cerebellum. Primary antibody: Anti-GABA Rabbit pAb (Cat. No. PC213L) (1:20,000). Detection: fluorescence.
DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. GABA (g-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system that is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
HostRabbit
Immunogen speciesRat
ImmunogenGABA
IsotypeIgG
Speciesrat
Positive controlRat thalamus or cerebellum
FormLyophilized
FormulationUndiluted serum.
PreservativeNone
CommentsAntibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.