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05-1139 Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7

This Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.

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Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: EC 2.7.10.2, FADK, FAK , FAK1 , pp125FAK UniProt Number: Q05397
05-1139
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, ChWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1139
Replaces04-591
DescriptionAnti-Focal Adhesion Kinase Antibody, clone BLAb2H7
Alternate Names
  • FADK 1
  • PTK2 protein tyrosine kinase 2
  • Protein-tyrosine kinase 2
  • focal adhesion kinase 1
Background InformationFAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al).
Product Information
FormatPurified
Control
  • HEK293 cell lysate
PresentationPurified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue.
Biological Information
ImmunogenRecombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553.
EpitopeTerminal
CloneBLAb2H7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes Focal Adhesion Kinase
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chicken
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EC 2.7.10.2
  • FADK
  • FAK
  • FAK1
  • pp125FAK
UniProt Number
UniProt SummaryFUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1 and STEAP4.
SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Molecular Weight~125 kDa
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
05-1139 04053252670091