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MABS351-I-100UG Anti-FAT10 Antibody, clone 4F1

Anti-FAT10, clone 4F1, Cat. No. MABS351-I, is a mouse monoclonal antibody that detects FAT10/Ubiquitin D and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MABS351-I-100UG
100 μg  
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Description
Catalogue NumberMABS351-I-100UG
DescriptionAnti-FAT10 Antibody, clone 4F1
Alternate Names
  • Ubiquitin D
  • UBD
  • Diubiquitin
Background InformationUbiquitin D (UniProt: O15205; also known as Diubiquitin, UBD, Ubiquitin-like protein FAT10) is encoded by the UBD (also known as FAT10) gene (Gene ID: 10537) in human. FAT10 is a ubiquitin-like modifier protein that is involved in various cellular processes, including apoptosis, cell cycle control, and NF- B activation. It is constitutively expressed in mature dendritic cells and B-cells. Its expression is observed in the reticuloendothelial system, the gastrointestinal system, kidney, lung, and prostate gland. It is detected in the nucleus as well as cytoplasm. It has two ubiquitin-like domains (aa 6-81 and 90-163). It can covalently attach to target protein and lead to their degradation by the 26S proteasome, in a NUB1-dependent manner. Its conjugation ability activated by UBA6. It regulates TNF -induced and LPS-mediated activation of NF- B by promoting TNF - mediated proteasomal degradation of I B . It is reported to mediate mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of mitotic arrest deficient 2 like 1 (MAD2L1) during the prometaphase stage. It mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and HIV-associated nephropathy. p62 protein that is involved in autophagy is covalently mono-FAT10ylated at several lysine residues, which leads to proteasomal degradation of p62. Prolonged induction of endogenous FAT10 by pro-inflammatory cytokines leads to a reduction in endogenous p62 levels. Clone 4F1 displays high-affinity for FAT10 and can immunoprecipitate monomeric FAT10 and FAT10-conjugated substrates. (Ref.: Aichem, A., et al. (2012). J. Cell Sci. 125(19); 4576-4585; Ren, J., et al. (2006). J. Biol. Chem. 281(16); 11413-11421; Ross, MJ., et al. (2006). J. Am. Soc. Nephrol. 17(4); 996-1004; Hipp, MS., et al. (2005). Mol. Cell Biol. 25(9); 3483-3491).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-FAT10, clone 4F1, Cat. No. MABS351-I, is a mouse monoclonal antibody that detects FAT10/Ubiquitin D and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesTested Applications

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected FAT10/Ubiquitin D in HepG2 cells.

Western Blotting Analysis: A representative lot detected FAT10/Ubiquitin D in Western Blotting application (Aichem, A., et al. (2012). J Cell Sci. 125(Pt19):4576-85; Aichem, A., et al. (2010). Nat Commun.;1:13).

Immunocytochemistry Analysis: A representative lot detected FAT10/Ubiquitin D in Immunocytochemistry applications (Aichem, A., et al. (2012). J Cell Sci. 125(Pt19):4576-85).

Immunoprecipitation Analysis: A representative lot immunoprecipitated FAT10/Ubiquitin D in Immunoprcipitation applications (Aichem, A., et al. (2012). J Cell Sci. 125(Pt19):4576-85).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenGST-tagged recombinant human FAT10/Ubiquitin D.
EpitopeUnknown
Clone4F1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 4F1 is a mouse monoclonal antibody that detects FAT10/Ubiquitin D.
IsotypeIgG2cκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • UBD
Purification MethodProtein G purified
UniProt Number
Molecular Weight~18 kDa observed; 18.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HepG2 cells treated with IFN and TNF .

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FAT10 in lysate from HepG2 cells treated with IFN and TNF (200 and 400 units, respectively for 16 h), but not in lysate from untreated HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS351-I-100UG 04065270451781