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MABS2190-25UG Anti-Extrahepatic biliary cells Antibody, clone hGB10

Anti-Extrahepatic biliary cells, clone hGB10, Cat. No. MABS2190, is a mouse monoclonal antibody that detects subset of gallbladder mucosal epitheliums with apical bias and is tested in Immunofluorescence and Immunohistochemistry (Paraffin).

MABS2190-25UG
25 μg  
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Description
Catalogue NumberMABS2190-25UG
DescriptionAnti-Extrahepatic biliary cells Antibody, clone hGB10
Alternate Names
  • EHBC
  • Gallbladder cells
Background InformationThe gallbladder is a small, distensible pouch-like organ that concentrates and stores bile produced by the liver that helps break down dietary fats. Bile flows out of the liver via hepatic ducts that join with the cystic duct, which conveys bile to and from the gall bladder. Gallbladder is derived from the foregut endoderm during embryonic development. In an un-distended state, its mucosa appears to be thrown into many folds. Its lumen is lined with a high columnar epithelium. This epithelial layer rests on a highly vascularized lamina propria. The biliary epithelium shows a morphological heterogeneity that is strictly associated with a variety of functions performed at the different levels of the biliary tree. These epithelial cells are also involved in absorption of water and inorganic salts and help to concentrate bile. The connective tissue wall of gallbladder contains abundant elastic fibers and layers of smooth muscles that run obliquely. Between the smooth muscle layers and serosa lies a thick layer subserosal layer of connective tissue. The availability of antibodies that preferentially mark cell populations within the extrahepatic biliary system are useful for identification and classification of gallbladder cells in relation to bile physiology, gallstone formation, cell plasticity and renewal, metaplasia, and tumor formation. Clone hBG10 is reported to specifically detect a subset of gallbladder mucosal epithelium with apical bias and peribiliary glands. (Ref.: Galivo, FH., et al. (2015). Stem Cell Res. 15(1); 172-181).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-Extrahepatic biliary cells, clone hGB10, Cat. No. MABS2190, is a mouse monoclonal antibody that detects subset of gallbladder mucosal epitheliums with apical bias and is tested in Immunofluorescence and Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected Extrahepatic biliary cells in Immunofluorescence applications (Galivo, F.H., et al. (2015). Stem Cell Res. 15(1):172-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenExtrahepatic human biliary cells
EpitopeUnknown
ClonehGB10
Concentration0.5 mg/ml. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone hGB10 is a mouse monoclonal antibody that detects a subset of mucosal epithelium with apical bias in human gallbladder.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human gallbladder tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected a subset of mucosal epithelium with apical bias and peribiliary glands in human gallbladder tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS2190-25UG 04065267463742