Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CBL419 Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: PRG2, MBP, BMPG, MGC14537 UniProt Number: P13727
CBL419
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, WB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL419
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Eosinophil Major Basic Protein Antibody, clone BMK13
Alternate Names
  • EMBP
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationThe monoclonal is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Quality LevelMQ100
Applications
ApplicationAnti-Eosinophil Major Basic Protein Antibody, clone BMK13 is an antibody against Eosinophil Major Basic Protein for use in ELISA, WB, IH(P).
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry: Clone BMK-13 reacts with fresh frozen tissues that were sectioned (6μm), dried for 1 hour at room temperature and fixed in 1:1 solution of methanol and acetone, and airdried for 1 hour. Sections can be stored wrapped in aluminium foil at -70°C until used. For paraffin embedded sections, 4% PFA (3hr) should be used for skin biopsies. 5 μM sections are dewaxed, and rehydrated with water. A proteinase treatment of 0.1% trypsin for 15 minutes in water should be performed prior to blocking and antibody staining.The antibody reacts better in frozen tissues than in paraffin embedded tissues.
Western blotting: purified normal human eosinophils were treated with digitonin (5mg/mL) for 10 minutes before boiling in sample buffer and run on a 15% SDS-PAGE. Purified MBP migrates at ~ 14kDa, and typical human samples range from 10-16kDa.
ELISA development for MBP estimation.

Optimal working dilutions must be determined by the end user.
Biological Information
CloneBMK13
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityCBL419 is specific for eosinophil major basic protein irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. This antibody will recognize the 23.8 kDa secreated pro-form of MBP (Pregnancy Associated MBP) found in the placenta and serum of pregnant women as well as the 13.8 kDa mature form of the protein which is found in the matrix of the eosinophil large specific granual. It does not cross-react with horse, mouse or rat MBP.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases.
Gene Symbol
  • PRG2
  • MBP
  • BMPG
  • MGC14537
Non-Reactive Species
  • Horse
  • Mouse
  • Rat
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P13727 # Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
SIZE: 222 amino acids; 25232 Da
SUBUNIT: In pregnancy serum, the proform exists as a disulfide- linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.
SUBCELLULAR LOCATION: Note=The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core).
TISSUE SPECIFICITY: High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.DEVELOPMENTAL STAGE: Levels of the proform increase in serum and placenta during pregnancy.
SIMILARITY: SwissProt: P13727 ## Contains 1 C-type lectin domain.
MISCELLANEOUS: Binds heparin. Does not bind calcium.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
CBL419 04053252516450

Documentation

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY - 2120959 2120959
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) MONOCLONAL ANTIBODY - 2356100 2356100
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3489746 3489746
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3519339 3519339
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3532422 3532422
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3543903 3543903
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3647969 3647969
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3674853 3674853
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3813498 3813498
MOUSE ANTI-HUMAN EOSINOPHIL MAJOR BASIC PROTEIN (MBP) - 3940602 3940602
Show more

References

Reference overviewPub Med ID
Age-related changes in proliferation, the numbers of mast cells, eosinophils, and cd45-positive cells in human dermis.
Gunin AG, Kornilova NK, Vasilieva OV, Petrov VV
The journals of gerontology Series A, Biological sciences and medical sciences  66  385-92. Epub 2010 Nov 24.  2010

Zobrazit abstrakt 		21106704 21106704