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205601 Anti-E-Cadherin Mouse mAb (HECD-1)

Anti-E-Cadherin Mouse mAb (HECD-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
205601
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.



We are offering Anti-E-cadherin Antibody, clone DECMA-1 (Cat. No. MABT26) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






Recognizes the E-cadherin protein.
Catalogue Number205601
Brand Family Calbiochem®
References
ReferencesJankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
Takeichi, M. 1991. Science 251, 1451.
Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.
Product Information
FormLyophilized
FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
Positive controlMCF-7 cells
PreservativeNone
Applications
Key Applications Cell Inhibition Assays
Competition Studies
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Immunofluorescence
Immunohistochemistry
Application NotesCompetition Studies (100 μg/ml)
ELISA (0.2-1 μg/ml)
Flow Cytometry (1 μg/ml)
Immunoblotting (2-10 μg/ml)
Immunofluorescence (1 μg/ml)
Immunohistochemistry (2-10 μg/ml)
Application CommentsMay be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal dilution.
Biological Information
ImmunogenMCF-7 human breast carcinoma cell line
ImmunogenHuman
CloneHECD-1
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with 50 μl dH₂O final concentration 2 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Note: Ca2+ should be present in all buffers to stabilize the cadherin antigen.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
205601 0

Documentation

Anti-E-Cadherin Mouse mAb (HECD-1) Certificates of Analysis

TitleLot Number
205601

References

Přehled odkazů
Jankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
Takeichi, M. 1991. Science 251, 1451.
Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-July-2007 RFH
ApplicationCompetition Studies (100 μg/ml)
ELISA (0.2-1 μg/ml)
Flow Cytometry (1 μg/ml)
Immunoblotting (2-10 μg/ml)
Immunofluorescence (1 μg/ml)
Immunohistochemistry (2-10 μg/ml)
DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-U1 mouse myeloma cells. Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the E-cadherin protein.
HostMouse
Immunogen speciesHuman
ImmunogenMCF-7 human breast carcinoma cell line
CloneHECD-1
IsotypeIgG₁
Specieshuman
Positive controlMCF-7 cells
FormLyophilized
FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
PreservativeNone
CommentsMay be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute with 50 μl dH₂O final concentration 2 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Note: Ca2+ should be present in all buffers to stabilize the cadherin antigen.
Toxicity Standard Handling
ReferencesJankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
Takeichi, M. 1991. Science 251, 1451.
Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.