Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE1905-100UG Anti-DNA ligase I Antibody, clone 5H5

Anti-DNA ligase I, clone 5H5, Cat. No. MABE1905, is a mouse monoclonal antibody that detects DNA ligase 1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABE1905-100UG
100 μg  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Catalogue NumberMABE1905-100UG
DescriptionAnti-DNA ligase I Antibody, clone 5H5
Alternate Names
  • DNA ligase 1
  • EC:6.5.1.1
  • Polydeoxyribonucleotide synthase [ATP] 1
Background InformationDNA ligase 1 (UniProt: P18858; also known as EC:6.5.1.1, DNA ligase I, Polydeoxyribonucleotide synthase [ATP] 1) is encoded by the LIG1 gene (Gene ID: 3978) in human. DNA ligase is the enzyme that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. DNA ligase I joins adjacent Okazaki fragments generated during the lagging strand synthesis and is recruited to replication factories during the S-phase of cell cycle. The recruitment is directed replication factory-targeting sequence (RFTS), which is also shown to mediate its interaction with the proliferating cell nuclear antigen (PCNA). Its C-terminal domain contains the catalytic region and consists of a DNA binding domain (aa 262-534), an adenylation domain (aa 535-747), and an OB-fold domain. The adenylation domain contains five conserved motifs (I, III, IIIa, IV, and V) that are reported to contribute to the surface of nucleotide binding pocket. The N-terminal region is non-catalytic and participates in nuclear localization and DNA replication. Human DNA ligase I can be phosphorylated on serine 66 by casein kinase 2 in a cell cycle-dependent manner. Several other phosphorylation sites have also been reported and the enzyme exits in a hyperphosphorylated state during the M-phase. (Ref.: Vitolo, B., et al. (2005). Eur. J. Histochem. 49(4); 349-354; Ferrari, G., et al. (2003). J. Biol. Chem. 278(39); 37761-3777; Howes, TR., and Tomkinson, AE. (2012). Subcell. Biochem. 62:327-41).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-DNA ligase I, clone 5H5, Cat. No. MABE1905, is a mouse monoclonal antibody that detects DNA ligase 1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected DNA ligase I in Immunohistochemistry applications (Vitolo, B., et. al. (2005). Eur J Histochem. 49(4): 349-54).

Western Blotting Analysis: A representative lot detected DNA ligase I in Western Blotting applications (Vitolo, B., et. al. (2005). Eur J Histochem. 49(4): 349-54).

Immunocytochemistry Analysis: A representative lot detected DNA ligase I in Immunocytochemistry applications (Vitolo, B., et. al. (2005). Eur J Histochem. 49(4): 349-54).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged purified human recombinant DNA ligase I overexpressed in a baculovirus/insect cell system.
Clone5H5
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5H5 is a mouse monoclonal antibody that detects human DNA ligase I.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Monkey based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LIGI
  • LIG1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~100 kDa observed; 101.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected DNA ligase I in HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE1905-100UG 04061841990185

Documentation

Anti-DNA ligase I Antibody, clone 5H5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-DNA ligase I Antibody, clone 5H5 Certificates of Analysis

TitleLot Number
Anti-DNA ligase I, clone 5H5 - 3749970 3749970
Anti-DNA ligase I, clone 5H5 - 3808667 3808667
Anti-DNA ligase I, clone 5H5 - 3862783 3862783
Anti-DNA ligase I, clone 5H5 - 3873606 3873606
Anti-DNA ligase I, clone 5H5 - 3878881 3878881
Anti-DNA ligase I, clone 5H5 - 3897612 3897612
Anti-DNA ligase I, clone 5H5 - 3905442 3905442
Anti-DNA ligase I, clone 5H5 - 3943303 3943303
Anti-DNA ligase I, clone 5H5 - 3943423 3943423
Anti-DNA ligase I, clone 5H5 - 4040797 4040797
Show more

References

Reference overviewPub Med ID
A new monoclonal antibody against DNA ligase I is a suitable marker of cell proliferation in cultured cell and tissue section samples
B Vitolo 1 , M R Lidonnici, C Montecucco, A Montecucco
Eur J Histochem  49(4):  349-54  2004

Zobrazit abstrakt 		16377576 16377576