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ABE2601-100UG Anti-DNA helicase B (HELB)

Anti-DNA helicase B (HELB) Antibody, Cat. No. ABE2601, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Western Blotting.

Anti-DNA helicase B (HELB) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HELB UniProt Number: Q8NG08
ABE2601-100UG
100 μg  
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Replacement Information

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2601-100UG
DescriptionAnti-DNA helicase B (HELB)
Alternate Names
  • DNA helicase B
  • hDHB
Background InformationDNA helicase B (UniProt: Q8NG08; also known as EC: 3.6.4.12, hDHB) is encoded by the HELB gene (Gene ID: 92797) in human. DNA helicase B is highly expressed in testis and thymus and weaker expression is observed in liver, spleen, kidney, and brain. It is predominantly nuclear, however, following phosphorylation at serine 967 by CDK2 during the G1/S transition it is exported into the cytoplasm. Following DNA damage, it is recruited to sites of double-strand breaks by the RPA complex. Its recruitment to single-stranded DNA following DNA damage leads to inhibition of nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following its phosphorylation. Its nuclear export signal is localized to amino acids 1055-1078. It uses either magnesium or manganese ions to support its helicase activity and the DNA helicase B activity is inhibited by salt concentrations higher than 100 mM. It binds strongly to single-stranded DNA in the absence of ATP, but dissociates readily in the presence of 1 mM ATP. Two isoforms of DNA helicase B have been reported that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-DNA helicase B (HELB) Antibody, Cat. No. ABE2601, is a highly specific rabbit polyclonal antibody that targets DNA helicase B (HELB) and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected DNA helicase B (HELB) in U2OS cells transfected with a non-targeting siRNA (CTRL) or siRNAs targeting protein and HeLa cells transfected with non-targeting (siCTRL) or HELB-targeting (siHELB) siRNAs (Tkac, J., et. al. (2016). Mol Cell. 61(3):405-418).
Biological Information
ImmunogenMBP-conjugated recombinant fragment corresponding to the first 199 amino acids from the N-terminal region of human DNA helicase B.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects DNA helicase B in human cells.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HELB
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~145 kDa observed; 123.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in siRNA control & siRNA HELB U2OS cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected DNA helicase B (HELB) in lysate from U2OS cells treated with non-targeting siRNA, but not in lysates from siRNA HELB treated U2OS cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE2601-100UG 04054839499470