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MAB8129 Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2

This Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Infectious Diseases Research Sub-Category: Infectious Diseases - Viral
MAB8129
100 µg  
Purchase on Sigma-Aldrich

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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, WB, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB8129
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytomegalovirus Antibody, immediate early, clone 7D12.2
Alternate Names
  • CMV
References
Product Information
FormatPurified
PresentationPurified ascites in 0.02 M Phosphate Buffered Saline, 0.25 M Sodium Chloride, 0.1% Sodium Azide,

pH 7.6 .
Quality LevelMQ300
Applications
ApplicationThis Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.
Key Applications
  • Immunofluorescence
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Applications Not Recommended
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry.

Immunocytochemistry.

IFA at 1:3,000-1:6,000 on acetone fixed cells.

Does not work on paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.
Biological Information
ImmunogenMRC-5 cells infected with CMV AD169.
Epitopeimmediate early
Clone7D12.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with an immediate early protein M.W. 68 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and cytoplasmic staining which reaches peak intensity between 8-24 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at +2° to + 8°C for up to 12 months from date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MAB8129 04053252612718

Documentation

Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 Certificates of Analysis

TitleLot Number
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN - 3679407 3679407
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2528628 2528628
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2538628 2538628
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2571294 2571294
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2573015 2573015
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2574667 2574667
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2576428 2576428
MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2836162 2836162