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MAB810-KC Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR)

Detect Cytomegalovirus using this Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR) validated for use in IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Infectious Diseases Research Sub-Category: Infectious Diseases - Viral
MAB810-KC
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB810-KC
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Cytomegalovirus Antibody, clone 8B1.2 (ASR)
OverviewThis product is intended for use by IVD manufacturers.
Contact customer service for assistance: oem.reagents@emdmillipore.com
Alternate Names
  • Anti-CMV
Background InformationHuman cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours (see figure below). After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication.
References
Product Information
FormatPurified
Control
  • Cytomegalovirus Control Slides, Catalogue Number 5027-5
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ300
Applications
ApplicationDetect Cytomegalovirus using this Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR) validated for use in IF.
Key Applications
  • Immunofluorescence
Application NotesELISA 1:800

Western Blot: 1:200. Recognizes approximately a 70kDa band.

Immunofluorescence: 1:200-1:400.

Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.

Works on paraffin embedded tissue sections.

FACS: See online protocol.

Final working dilutions and protocols must be determined by end user.
Biological Information
ImmunogenAffinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Clone8B1.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Purification MethodProtein A Purfied
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Availability by Geography
  • This product is only available for sale in the United States and Canada.
Usage Statement
  • Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material SizeBulk packaging. Please inquire.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MAB810-KC 04053252378195