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MABF2108-100UG Anti-Chlamydial HSP60 Antibody, clone A57-B9

Anti-Chlamydial HSP60, clone A57-B9, Cat. No. MABF2108, is a mouse monoclonal antibody that detects Chlamydial HSP60 and has been tested for use in ELISA, Immunofluorescence, Immunocytochemistry, and Western Blotting.

MABF2108-100UG
100 μg  
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Description
Catalogue NumberMABF2108-100UG
DescriptionAnti-Chlamydial HSP60 Antibody, clone A57-B9
Alternate Names
  • Chlamydia heat shock protein 60
Background InformationChlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV). Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants. These are identified based on serological reactivity of the epitopes on their outer membrane. Intracellularly chlamydia replicates within a vacuole. Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles. This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell. Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate. Clone A57-B9 detects Chlamydial HSP60 (GroEL). It reacts with the short peptide of 6 amino acids from the carboxyl terminal region of HSP60. HSP60 facilitates the correct folding of imported proteins and may prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Chlamydial HSP60, clone A57-B9, Cat. No. MABF2108, is a mouse monoclonal antibody that detects Chlamydial HSP60 and has been tested for use in ELISA, Immunofluorescence, Immunocytochemistry, and Western Blotting.
Key Applications
  • ELISA
  • Immunofluorescence
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).

Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).

ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).

Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Biological Information
ImmunogenRecombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
CloneA57-B9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
IsotypeIgG1κ
Species Reactivity
  • Bacteria
Species Reactivity NoteBacteria (Chlamydia genus)
Antibody TypeMonoclonal Antibody
Gene Symbol
  • N/A
Purification MethodProtein G purified
Molecular Weight~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysates from HeLa cells infected with chlamydia trachomatis serovar L2 (LGV 434 L2) .

Western Blotting Analysis: 2 µg/mL of this antibody detected Chlamydial HSP60 in lysates from HeLa cells infected with Chlamydia trachomatis serovar L2 (LGV 434 L2) .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABF2108-100UG 04054839636455