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AM46T Anti-Caspase-8 (Ab-3) Mouse mAb (1-3)

Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CAP4, Anti-CASP8, Anti-FLICE, Anti-ICE-LAP5, Anti-MACH, Anti-Mch5

AM46T
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Reacts with the zymogen form of caspase-8 (both reported forms; 55-58 kDa in non-apoptotic Jurkat cells) and with the 18 kDa subunit in apoptotic Jurkat or HeLa cells. Additional bands at 42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines.
Catalogue NumberAM46T
Brand Family Calbiochem®
SynonymsAnti-CAP4, Anti-CASP8, Anti-FLICE, Anti-ICE-LAP5, Anti-MACH, Anti-Mch5
References
ReferencesFearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Juo, P., et al. 1998. Curr. Biol. 8, 1001.
Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
Peter, M.E., and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
Sakamaki, K, et al. 1998. Eur. J. Biochem. 253, 399.
Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
Muzio, M., et al. 1996. Cell 85, 817.
Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
Positive controlHeLa or Jurkat cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Not Frozen Sections
Not Immunofluorescence
Not Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin Sections (not recommended)
Application CommentsAdditional bands at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human caspase-8
ImmunogenHuman
Clone1-3
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
AM46T 0

Documentation

Anti-Caspase-8 (Ab-3) Mouse mAb (1-3) Certificates of Analysis

TitleLot Number
AM46T

References

Přehled odkazů
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Juo, P., et al. 1998. Curr. Biol. 8, 1001.
Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
Peter, M.E., and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
Sakamaki, K, et al. 1998. Eur. J. Biochem. 253, 399.
Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
Muzio, M., et al. 1996. Cell 85, 817.
Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.

Citations

Název
  • Cecile Tetaud, et al. (2003) Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1. Journal of Biological Chemistry 278, 45200-45208.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-August-2007 RFH
    SynonymsAnti-CAP4, Anti-CASP8, Anti-FLICE, Anti-ICE-LAP5, Anti-MACH, Anti-Mch5
    ApplicationImmunoblotting (1 µg/ml)
    Frozen Sections (not recommended)
    Immunofluorescence (not recommended)
    Immunoprecipitation (not recommended)
    Paraffin Sections (not recommended)
    DescriptionPurified mouse monoclonal antibody. Recognizes the ~56-58 kDa latent and the ~28 kDa active forms of caspase-8.
    BackgroundCaspase-8 (ICE-LAP5, MACH, FLICE, CAP4) is the most proximal of the members of the apoptotic caspase cascade. Caspase-8 is directly involved in apoptosis mediated through either Fas or the TNF receptor and forms one of the key components of the transducing DISC, the CAP4 protein. Cell lines that are deficient in caspase-8 are resistant to Fas mediated apoptosis. Nine different isoforms of caspase-8 have been identified but the "a" and "b" isoforms show the highest expression at the level of protein in multiple tissues. Caspase-8 has a pH optimum of 6.8 and has a kcat/Km of 8.7 X 105 M-1 s-1. The pro-form of caspase-8 has a molecular weight of ~55 kDa and undergoes several processing steps to generate the active heterodimer of p18 and p10/12. In addition to activation via Fas, oligomerization of caspase-8 at the cell membrane and interaction with Granzyme B and caspase-10 will induce auto-processing and activation of the apoptotic pathway. The mouse caspase-8 gene has also been identified and is expressed in the adult as well as at high levels in the day 9.5 and day 17.5 embryo suggesting a role in development.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant human caspase-8
    Clone1-3
    IsotypeIgG₁
    Specieshuman
    Positive controlHeLa or Jurkat cells
    Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
    Concentration Label Please refer to vial label for lot-specific concentration
    PreservativeNone
    CommentsAdditional bands at ~42-44 kDa corresponding to the heavier processed chain may appear in some apoptotic cell lines. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesFearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
    Juo, P., et al. 1998. Curr. Biol. 8, 1001.
    Martin, D.A., et al. 1998. J. Biol. Chem. 273, 4345.
    Peter, M.E., and Krammer, P.H. 1998. Curr. Opin. Immunol. 10, 545.
    Sakamaki, K, et al. 1998. Eur. J. Biochem. 253, 399.
    Stennicke, H.R., et al. 1998. J. Biol. Chem. 273, 27084.
    Scaffifi, C., et al. 1997. J. Biol. Chem. 272, 26953.
    Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
    Muzio, M., et al. 1996. Cell 85, 817.
    Kischkel, F.C., et al. 1995. EMBO J. 14, 5579.
    Citation
  • Cecile Tetaud, et al. (2003) Two Distinct Gb3/CD77 Signaling Pathways Leading to Apoptosis are Triggered by Anti-Gb3/CD77 mAb and Verotoxin-1. Journal of Biological Chemistry 278, 45200-45208.
    		•