Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABT283 Anti-Cadherin-11/OB Cadherin Antibody

This Anti-Cadherin-11/OB Cadherin Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Cadherin-11/OB Cadherin.

Anti-Cadherin-11/OB Cadherin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CDH11 UniProt Number: P55287
ABT283
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT283
DescriptionAnti-Cadherin-11/OB Cadherin Antibody
Alternate Names
  • Cadherin-11
  • OSF-4
  • Osteoblast cadherin
  • OB-cadherin
Background InformationCadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationPurified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Cadherin-11/OB Cadherin Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Cadherin-11/OB Cadherin.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 2 µg/mL of a representative lot of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Mouse Brain, Mouse Brain Cytosol, Mouse Brain Membrane, Mouse Brain Nuclear Extract, Mouse (Aged) Brain Frontal Cortex, and Mouse Cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution of a representative lot of this antibody detected Cadherin-11/OB Cadherin in neurons of the human cerebral cortex and thalamus, as well as purkinje and basket cells in human cerebellum.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the Cadherin 5 domain of Human Cadherin-11/OB Cadherin.
EpitopeCadherin 5 domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDH11
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~75 kDa observed.
Full length protein has a molecular weight of ~120 kDa, however the cleaved product has a molecular weight of ~75-80 kDa (McCusker, C., et al. (2009) MBC. 20(1):78-89). Uncharacterized bands may be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human Thalamus Tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Human Thalamus Tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABT283 04053252996146