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ST1548 Anti-CTH Mouse mAb (S51)

Anti-CTH Mouse mAb (S51) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cystathionase, Anti-Cystathionase γ-Lyase

ST1548
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~38 kDa CTH protein in K-562 cells and human pancreas tissue.
Catalogue NumberST1548
Brand Family Calbiochem®
SynonymsAnti-Cystathionase, Anti-Cystathionase γ-Lyase
Application Data
Detection of human CTH by immunoblotting. Sample: Whole cell lysate from K-562 cells. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CTH by staining praffin sections. Sample: Formalin-fixed paraffin embedded human pancreas tissue. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (3 µg/ml). Detection: colorimetric (DAB).
References
ReferencesZhu, X.Y., et al. 2010. Cell Mol Life Sci. 67, 1119.
Siebert, N., et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G1266.
Product Information
FormLiquid
Formulationin PBS, pH 7.2
Negative control293T cells
Positive controlK-562 cells, Human pancreas tissue
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant human CTH (aa 1-406) expressed as a GST fusion protein
CloneS51
HostMouse
IsotypeIgG1
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ST1548 0

Documentation

References

Přehled odkazů
Zhu, X.Y., et al. 2010. Cell Mol Life Sci. 67, 1119.
Siebert, N., et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G1266.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Cystathionase, Anti-Cystathionase γ-Lyase
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human CTH by immunoblotting. Sample: Whole cell lysate from K-562 cells. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CTH by staining praffin sections. Sample: Formalin-fixed paraffin embedded human pancreas tissue. Priamry antibody: Anti-CTH Mouse mAb (S51) (Cat. No. ST1548) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~38 kDa CTH protein.
BackgroundCTH is a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria.
HostMouse
ImmunogenFull-length, recombinant human CTH (aa 1-406) expressed as a GST fusion protein
CloneS51
IsotypeIgG1
Specieshuman
Positive controlK-562 cells, Human pancreas tissue
Negative control293T cells
FormLiquid
Formulationin PBS, pH 7.2
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesZhu, X.Y., et al. 2010. Cell Mol Life Sci. 67, 1119.
Siebert, N., et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G1266.